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인체 대장종양에서의 새로운 Heat Shock Protein의 발현

Expression of a Novel 90 kDa Heat Shock Protein in Colorectal Tumor

대한대장항문학회지 2002년 18권 1호 p.1 ~ 6
최대화 ( Choi Dae-Hwa ) - 울산대학교 의과대학 울산대학교병원 외과

조홍래 ( Cho Hong-Rae ) - 울산대학교 의과대학 울산대학교병원 외과학교실
고병균 ( Ko Byung-Kyun ) - 울산대학교 의과대학 울산대학교병원 외과
나양원 ( Nah Yang-Won ) - 울산대학교 의과대학 울산대학교병원 외과
남창우 ( Nam Chang-Woo ) - 울산대학교 의과대학 울산대학교병원 외과학교실
김규열 ( Kim Kyu-Yeol ) - 울산대학교 의과대학 울산대학교병원 외과
임영철 ( Lim Young-Chul ) - 울산대학교 의과대학 울산대학교병원 외과학교실
박건춘 ( Park Kun-Choon ) - 울산대학교 의과대학 외과학교실
김도하 ( Kim Do-Ha ) - 울산대학교병원 내과
박재후 ( Park Jae-Hoo ) - 울산대학교병원 내과
민영주 (  ) - 울산대학교 의과대학 내과학교실
서재희 ( Suh Jae-Hee ) - 울산대학교 의과대학 울산대학교병원 진단병리학교실
박정우 ( Park Jeong-Woo ) - 울산대학교 자연과학대학 생명과학부

Abstract


Purpose: When cells are subjected to a wide variety of stressful stimuli, they respond by increasing the synthesis of specific stress proteins. Stresses include heat shock, nutrient deprivation, oxygen radicals, toxic metal and viral
infection. Major stress proteins are Hsp 27, Hsp 60, Hsp 70 (9), Hsp 90 (3) and Hsp 100 (1). Previously a novel 90 kDa stress protein has been reported to be induced in fish cells by virus infection. The novel 90 kDa stress protein is different from well-known major stress protein in size, antigenicity, cellular localization. The novel 90 kDa stress protein was found to be present in various kinds of cells including human cells and its expression was increased in human carcinomas. The purpose of this study is to evaluate the expression of the novel 90 kDa stress protein in human colonic mucosa of normal tissue, adenoma & adenocarcinoma using immunohistochemical method.

Methods: 85 colon tissues were screened for the expression of the novel 90 kDa stress protein; 85 normal colonic mucosa, 20 colonic adenoma and 65 colonic adenocarcinoma. The tissues were stained with monoclonal antibody against the novel 90 kDa stress protein. In scoring system, tissue sections with immunostained area above 10 % were decided to be positive and, among the positive, the tissue sections were divided into three score, 1, 2, and 3, based on the staining intensity and positive area proportion. The tissue sections with immunostained area below 10% were decided
to be negative and grouped into 0 score. Correlation of immunohistochemical expression was analysed by using SPSS version 10.0 statistically.

Results: The expression of the 90 kDa stress protein was significantly different among normal colonic
mucosa, colonic adenoma, and colonic adenocarcinoma and the percentage of positive samples were 14.1%, 80.5%, and 95.4% respectively. This result suggests that the expression level of the novel 90 kDa stress protein was extremely low in normal tissue but increased significantly in adenocarcinomatous tissues.

Conclusions: The expression of the novel 90 kDa stress protein was increased significantly with transformation of the normal colon tissue to malignancy. This suggests the possibility that this novel 90 kDa stress protein play some role in cancerous transformation of colon tissue.

키워드

열쇽단백;결직장 선종;결직장 선암종
Heat shock protein;Colorectal adenoma;Colorectal adenocarcinoma
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