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우측 대장암 환자 문맥혈에서의 Cytokeratin 19 및 20의 발현

Detection of Cytokeratin 19 and 20 in Portal Bloods in Right Colon Cancer Patients

대한대장항문학회지 2003년 19권 5호 p.322 ~ 326
김홍 ( Kim Hong ) - 아주대학교 의과대학 외과학교실

김봉완 ( Kim Bong-Wan ) - 아주대학교 의과대학 외과학교실
김혜진 ( Kim Hae-Jin ) - 아주대학교 의과대학 외과학교실
서광욱 ( Suh Kwang-Wook ) - 아주대학교 의과대학 외과학교실

Abstract


Purpose: We planned this prospective study to detect tumor cells in portal venous blood during a curative operation for colon cancer and to identify its clinical implications.

Methods: From August to December 1998, we collected portal venous blood (10 ml) during curative operations on 20 patients with colon cancer. Cytokeratin (CK) 19 and 20 transcripts were amplified using a reverse transcriptase-PCR assay. As a negative control, 10 ml of portal blood from 10 patients who underwent benign surgery were assayed. The HCT-116 colon cancer cell line was used for the positive control. All patients were closely followed until May 2003 (mean follow-up: 55 months).

Results: CK 19 was positive in 17 (85.0%) patients, and CK 20 was positive in 6 (30.0%) patients. However, CK 19 was also detected in 8 (66.7%) control patients, whereas CK 20 was negative in all control patients. CK 20 was found to be more relevant to the pathologic stage. During the follow-up period, liver metastases were found in 3 patients (50%) who had shown CK 20 in their portal bloods.

Conclusion: CK 20 is found to be more specific than CK 19 in the detection of epithelial cells from portal blood. Moreover, CK 20 is related to the stage and was predictive of hepatic metastases in 50 % of the patients with colon cancer. With further accumulation of cases, the prognostic significance of CK 20 for hepatic metastases may be better evaluated.

키워드

역전사 다중효소중합 반응;사이토케라틴 19;사이토케라틴 20;문맥;결장종양
Cytokeratin;Reverse transcriptase polymerase chain reaction;Colonic neoplasms;Portal vein
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