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대장의 증식성 용종 환자에서 바이오칩을 이용한 유전자 발현의 초기 선험적 연구

A Pilot Study as the Biochip Based Gene Expression Profiling in Patients with Hyperplastic Colonic Polyp

대한대장항문학회지 2006년 22권 4호 p.241 ~ 249
박웅채 ( Park Ung-Chae ) - 건국대학교 의과대학 외과학교실

김경래 ( Kim Kyung-Rae ) - 건국대학교 의과대학 외과학교실
성무경 ( Seong Moo-Kyung ) - 건국대학교 의과대학 외과학교실
왕준호 ( Wang Joon-Ho ) - 건국대학교 의과대학 내과학교실
이재동 ( Lee Jae-Dong ) - 건국대학교 의과대학 내과학교실
김상윤 ( Kim Sang-Yoon ) - 건국대학교 의과대학 병리학교실
박승화 ( Park Seung-Hwa ) - 건국대학교 의과대학 해부학교실
최동국 ( Choi Dong-Kug ) - 건국대학교 의료생명대학 생명공학과
김찬길 ( Kim Chan-Gil ) - 건국대학교 생명공학과

Abstract


Purpose: A microarray-based gene expression analysis may offer a rapid and efficient means for assessing. However, the molecular genetic change in nonneoplastic colonic polyp is still poorly understood. To elucidate the molecular genetic basis, We now report the results of our initial microarray data to analyze the genom pattern in patients with hyperplastic polyps of colon.

Methods: 36 samples (18 pairs of colonic polyps and normal colonic mucosa were) harvested from colonoscopic biopsy. 3 of 18 colonic polyps were pathologically identified as the serrated type of hyperplastic polyp. We used the oligonucleotide microarray technique for analysis of the expression profiles of serrated polyps and normal mucosa. For the identification of differentially expressed genes, SAM (Significance Analysis of Microarray) package method was used. The result was analysed by using global normalization, intensity dependent normalization and block-wise normalization.

Results: Polypectomy specimens microscopically showed the pathologically characteristic serration with a saw-teeth like luminal border (branching of the crypts). 8 genes including RHEB (Ras homolog enriched in brain), WASF2 (WAS protein family, member 2), TYRP1 (Tyrosinase-related protein 1), VSX1 (Visual system homeobox 1 homolog), ROS1 (V-ros UR2 sarcoma virus oncogene homolog 1), WEE1 (WEE1 homolog), TEC (Tec protein tyrosine kinase), TNFRSF10A (Tumor necrosis factor receptor superfamily, member 10a) in serrated polyp were up-regulated by more than 10 times as compared with normal colonic mucosa. On the other hand, 6 genes including SIAT7D (Sialyltransferase 7D), DRD1 (Dopamine receptor D1), SIAT1 (Sialyltransferase 1), ITSN1 (Intersectin 1), TNFSF12 (Tumor necrosis factor superfamily, member 12), CHES1 (Checkpoint suppressor 1) were down-regulated by less than a tenth of the expression as compared with normal colonic mucosa.

Conclusions: Serrated polyps as a subset of hyperplastic colonic polyps were analyzed with the oligonucleotide microarray technique. We authors could identify 14 genes (8 up-regulated and 6 down-regulated genes) that showed the significant change of expression as compared with normal colonic mucosa. Specifically, we believe that current study will serve as a fundamental base to offer a bioinformative characteristics of the serrated colonic polyp in future clinical applications. J Korean Soc Coloproctol 2006;22:241-249

키워드

대장 증식성 용종;거치상 용종;유전자 발현
Hyperplastic colonic polyp;Serrated polyp;Gene expression
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