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Adipose-tissue-derived Stem Cells Enhance the Healing of Ischemic Colonic Anastomoses: An Experimental Study in Rats

대한대장항문학회지 2012년 28권 3호 p.132 ~ 139
 ( Yoo Jong-Han ) - Inje University College of Medicine Busan Paik Hospital Department of Surgery

 ( Shin Jae-Ho ) - Inje University College of Medicine Busan Paik Hospital Department of Surgery
 ( An Min-Sung ) - Inje University College of Medicine Busan Paik Hospital Department of Surgery
 ( Ha Tae-Kwun ) - Inje University College of Medicine Busan Paik Hospital Department of Surgery
 ( Kim Kwang-Hee ) - Inje University College of Medicine Busan Paik Hospital Department of Surgery
 ( Bae Ki-Beom ) - Inje University College of Medicine Busan Paik Hospital Department of Surgery
 ( Kim Tae-Hyeon ) - Inje University College of Medicine Busan Paik Hospital Department of Surgery
 ( Choi Chang-Soo ) - Inje University College of Medicine Busan Paik Hospital Department of Surgery
 ( Hong Kwan-Hee ) - Inje University College of Medicine Busan Paik Hospital Department of Surgery
김정 ( Kim Jeong ) - Inje University Busan Paik Hospital Department of Pathology
 ( Jung Soo-Jin ) - Inje University Busan Paik Hospital Department of Pathology
 ( Kim Sun-Hee ) - Inje University Paik Institute for Clinical Research
 ( Rho Kuk-Hwan ) - Inje University Paik Institute for Clinical Research
 ( Kim Jong-Tae ) - Inje University Paik Institute for Clinical Research
양영일 ( Yang Young-Il ) - Inje University Busan Paik Hospital Department of Pathology

Abstract


Purpose: This experimental study verified the effect of adipose-tissue-derived stem cells (ASCs) on the healing of ischemic colonic anastomoses in rats.

Methods: ASCs were isolated from the subcutaneous fat tissue of rats and identified as mesenchymal stem cells by identification of different potentials. An animal model of colonic ischemic anastomosis was induced by modifying Nagahata’s method. Sixty male Sprague-Dawley rats (10-week-old, 370 ± 50 g) were divided into two groups (n = 30 each): a control group in which the anastomosis was sutured in a single layer with 6-0 polypropylene without any treatment and an ASCtreated group (ASC group) in which the anastomosis was sutured as in the control group, but then ASCs were locally transplanted into the bowel wall around the anastomosis. The rats were sacrificed on postoperative day 7. Healing of the anastomoses was assessed by measuring loss of body weight, wound infection, anastomotic leakage, mortality, adhesion formation, ileus, anastomotic stricture, anastomotic bursting pressure, histopathological features, and microvascular density.

Results: No differences in wound infection, anastomotic leakage, or mortality between the two groups were observed. The ASC group had significantly more favorable anastomotic healing, including less body weight lost, less ileus, and fewer ulcers and strictures, than the control group. ASCs augmented bursting pressure and collagen deposition. The histopathological features were significantly more favorable in the ASC group, and microvascular density was significantly higher than it was in the control group.

Conclusion: Locally-transplanted ASCs enhanced healing of ischemic colonic anastomoses by increasing angiogenesis. ASCs could be a novel strategy for accelerating healing of colonic ischemic risk anastomoses.

키워드

Colonic anastomosis; Ischemia; Anastomotic healing; Adipose-tissue-derived stem cell; Angiogenesis
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