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쥐의 과립막 세포 자연사에 EGF, TGF-α가 미치는 영향

Regulation of the apoptosis on the rat granulosa cell: effects of EGF, TGF-α

대한산부인과학회지 2004년 47권 12호 p.2439 ~ 2446
조현희/Jo HH
황성진/김현영/김미란/권동진/김장흡/김진홍/Hwang SJ/Kim HY/Kim MR/Kwon DJ/Kim JH/Kim JH

Abstract


Objective: To evaluate the effect of the epidermal growth factor (EGF) and the transforming growth factor-alpha (TGF-alpha) on the apoptosis of the rat granulosa cells.

Methods: For this study, granulosa cells were isolated from female rats, and granulosa cells were cultured in the presence or absence of different autocrine/paracrine factors such as EGF and TGF-alpha. In order to investigate the apoptotic process, the number of apoptotic cells were counted using Annexin V staining via flow cytometry. In addition, the expression levels of the Bcl-2, ICE and BAX genes were checked.

Results: c-AMP induced apoptosis was inhibited neither by EGF nor by TGF-alpha, but FSH induced apoptosis was inhibited by EGF and TGF-alpha. Both EGF and TGF-alpha decreased the rate of apoptosis in the serum free cultured media in a time dependent manner by inducing survival gene expression such as Bcl-2 and suppressing death gene expression such as ICE and BAX.

Conclusion: EGF and TGF-alpha can be used to decrease the apoptotic rate of the rat granulosa cells. It is hoped to conduct a similar study on the function of these growth factors in the human granulosa cells, and to use EGF and TGF-alpha for reducing the apoptosis rate of the human granulosa cells in IVF.

키워드

Granulosa cells;Epidermal growth factor;Transforming growth factor;Apoptosis;세포 자연사;EGF;TGF-α
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