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Glutamate dehydrogenase antigen detection in Plasmodium falciparum infections

Korean Journal of Parasitology 1996년 34권 4호 p.239 ~ 253

Abstract


The usefulness of malaria diagnosis by Plasmodium falciparum-GDH(NADP+), obtained by affinity chromatography, is demonstrated in ELISA assays, testing IgG antibodies against CDH(NADP+) from patients with acute malaria. Who have had two or
more
episodes of malaria. Or from sera of hyperimmune patients. CDH(NADP+) thermal stability was demonstrated in a high heat resistance assay. The immunofluorescence assay demonstrated that anti-culture (P. falciparum) supernatant serum and
anti-GDH(NADP+)
of Proteus spp, recognized epitopes in Venezulan isolates, and Colombian and Brasilian malarialstrains. The antigen is soluble, with high specificity, is a potent immunogen and is thermoresistant.

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