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Reactivity of German Cockroach Allergen, Bla g 2, Peptide Fragments to IgE Antibodies in Patients’ Sera

Korean Journal of Parasitology 2008년 46권 4호 p.243 ~ 246
 ( Lee Hae-Seok ) - 연세대학교 의과대학 환경의생물학교실

홍천수 ( Hong Chein-Soo ) - 연세대학교 의과대학 내과학교실
 ( Jeong Kyoung-Yong ) - 연세대학교 의과대학 환경의생물학교실
 ( Shin Kwang-Hyun ) - 연세대학교 의과대학 환경의생물학교실
 ( Yi Myung-Hee ) - 연세대학교 의과대학 환경의생물학교실
 ( Gantulaga Darambazar ) - 몽골 Health Science University Department of Medical Biology
용태순 ( Yong Tai-Soon ) - 연세대학교 의과대학 환경의생물학교실

Abstract


Bla g 2 is a cockroach allergen of great importance. This study was conducted to identify IgE-binding epitope(s) of Bla g 2 using the recombinant protein technique. Approximately 50% of tested sera showed IgE reactivity to Pichia-expressed Bla g 2 (PrBla g 2) and E. coli-expressed Bla g 2 (ErBla g 2). Only 5.3% of serum samples showed stronger reactivity to PrBla g 2 than ErBla g 2, indicating that serum was reactive to conformational or carbohydrate epitopes. The full-length and 5 peptide fragments of Bla g 2 were produced in E. coli. All fragments showed IgE-binding activity to the cockroach-allergy patients’ sera. Specifically, peptide fragments of amino acid residue 1-75 and 146-225 appeared to be important for IgE-binding. The information about the IgE-binding epitope of Bla g 2 can aid in the diagnosis and treatment for cockroach allergies.

키워드

Blattella germanica;cockroach;Bla g 2;allergen;epitope
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