잠시만 기다려 주세요. 로딩중입니다.

Genetic Variability of Antigen B among Echinococcus granulosus Egyptian Isolates

Korean Journal of Parasitology 2009년 47권 3호 p.259 ~ 264
 ( Tawfeek Gihan M. ) - Ain Shams University Faculty of Medicine Department of Parasitology

 ( Elwakil Hala S. ) - Ain Shams University Faculty of Medicine Department of Parasitology
 ( Awad Nabil S. ) - Ain Shams University Centre for Genetic Engineering & Biotechnology
 ( Laila El-Hoseiny ) - Ain Shams University Faculty of Medicine Department of Parasitology
 ( Thabet Hala S. ) - Ain Shams University Faculty of Medicine Department of Parasitology
 ( Sarhan Rania M. ) - Ain Shams University Faculty of Medicine Department of Parasitology
 ( Darweesh Samar K. ) - Cairo University Kasr El-Aini Hospital
 ( Anwar Wagida A. ) - Ain Shams University Centre for Genetic Engineering & Biotechnology

Abstract


Genetic polymorphisms of encoding antigen B2 gene (AgB2) in Echinococcus granulosus were studied using PCR-RFLP and DNA sequencing among 20 Egyptian isolates. Five isolates from different host origins (humans, camels, pigs, and sheep) were collected and used. All examined isolates of each host group gave very similar patterns of PCR-RFLP after restriction enzyme digestion with AluI, with the gene size of approximately 140 bp and 240 bp for sheep and human isolates, and approximately 150 bp and 250 bp for pig and camel isolates. No digestion pattern was obtained after incubation of all studied isolates with EcoRI. These results reveal high intra-group homogeneity. DNA sequence analysis highlighted that human infecting strain showed 100% identity with respect to sheep infecting isolate, 96% and 99% with pig and camel infecting isolates, respectively.

키워드

Echinococcus granulosus;AgB polymorphism;genetic diversity;PCR-RFLP;riboprinting pattern;gene sequence
원문 및 링크아웃 정보
  
등재저널 정보
SCI(E)
MEDLINE
KCI
KoreaMed
KAMS