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Identification of Antigenic Proteins in Trichomonas vaginalis

Korean Journal of Parasitology 2011년 49권 1호 p.79 ~ 83
 ( Lee Hye-Yeon ) - Yonsei University College of Medicine Department of Environmental Medical Biology

 ( Hyung Su-Jin ) - Yonsei University College of Medicine Department of Environmental Medical Biology
 ( Lee Jong-Woong ) - Yonsei University College of Medicine Department of Environmental Medical Biology
 ( Kim Ju-Ri ) - Yonsei University College of Medicine Department of Environmental Medical Biology
 ( Shin Myeong-Heon ) - Yonsei University College of Medicine Institute of Tropical Medicine
 ( Ryu Jae-Sook ) - Hanyang University College of Medicine Department of Environmental Medical Biology
 ( Park Soon-Jung ) - Yonsei University College of Medicine Department of Environmental Medical Biology

Abstract


Trichomoniasis is a sexually transmitted disease due to infection with Trichomonas vaginalis, and it can cause serious consequences for women’s health. To study the virulence factors of this pathogen, T. vaginalis surface proteins were investigated using polyclonal antibodies specific to the membrane fractions of T. vaginalis. The T. vaginalis expression library was constructed by cloning the cDNA derived from mRNA of T. vaginalis into a phage λ Uni-ZAP XR vector, and then used for immunoscreening with the anti-membrane proteins of T. vaginalis antibodies. The immunoreactive proteins identified included adhesion protein AP65-1, α-actinin, kinesin-associated protein, teneurin, and 2 independent hypothetical proteins. Immunofluorescence assays showed that AP65-1, one of the identified immunogenic clones, is prevalent in the whole body of T. vaginalis. This study led us to identify T. vaginalis proteins which may stimulate immune responses by human cells.

키워드

Trichomonas vaginalis; antigenic protein; membrane protein; immunoscreening
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