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CD8+ T-cell Activation in Mice Injected with a Plasmid DNA Vaccine Encoding AMA-1 of the Reemerging Korean Plasmodium vivax

Korean Journal of Parasitology 2011년 49권 1호 p.85 ~ 90
 ( Kim Hyo-Jin ) - Seoul National University College of Medicine Department of Parasitology and Tropical Medicine

 ( Jung Bong-Kwang ) - Seoul National University College of Medicine Department of Parasitology and Tropical Medicine
 ( Lee Jin-Joo ) - Seoul National University College of Medicine Department of Parasitology and Tropical Medicine
 ( Pyo Kyoung-Ho ) - Seoul National University College of Medicine Department of Parasitology and Tropical Medicine
 ( Kim Tae-Yun ) - Yonsei University College of Medicine Department of Environmental Medical Biology
 ( Choi Byung-Il ) - Korea University College of Medicine Department of Anatomy
 ( Kim Tae-Woo ) - Korea University Graduate School of Medicine Divison of Infection and Immunology
 ( Hisaeda Hajime ) - Gunma University Graduate School of Medicine Department of Parasitology
 ( Himeno Kunisuke ) - Kyushu University Graduate School of Medical Sciences Department of Parasitology
 ( Shin Eun-Hee ) - Seoul National University College of Medicine Department of Parasitology and Tropical Medicine
 ( Chai Jong-Yil ) - Seoul National University College of Medicine Department of Parasitology and Tropical Medicine

Abstract


Relatively little has been studied on the AMA-1 vaccine against Plasmodium vivax and on the plasmid DNA vaccine encoding P. vivax AMA-1 (PvAMA-1). In the present study, a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax has been constructed and a preliminary study was done on its cellular immunogenicity to recipient BALB/c mice. The PvAMA-1 gene was cloned and expressed in the plasmid vector UBpcAMA-1, and a protein band of approximately 56.8 kDa was obtained from the transfected COS7 cells. BALB/c mice were immunized intramuscularly or using a gene gun 4 times with the vaccine, and the proportions of splenic T-cell subsets were examined by fluorocytometry at week 2 after the last injection. The spleen cells from intramuscularly injected mice revealed no significant changes in the proportions of CD8+ T-cells and CD4+ T-cells. However, in mice immunized using a gene gun, significantly higher (P<0.05) proportions of CD8+ cells were observed compared to UB vector-injected control mice. The results indicated that cellular immunogenicity of the plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax was weak when it was injected intramuscularly; however, a promising effect was observed using the gene gun injection technique.

키워드

Plasmodium vivax; DNA vaccine; apical membrane antigen (AMA); CD8+ T-cell; gene gun
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