잠시만 기다려 주세요. 로딩중입니다.

A 24 kDa Excretory-Secretory Protein of Anisakis simplex Larvae Could Elicit Allergic Airway Inflammation in Mice

Korean Journal of Parasitology 2011년 49권 4호 p.373 ~ 380
 ( Park Hye-Kyung ) - Pusan National University College of Medicine Department of Internal Medicine

 ( Cho Min-Kyoung ) - Pusan National University School of Medicine Department of Parasitology
 ( Park Mi-Kyung ) - Pusan National University School of Medicine Department of Parasitology
 ( Kang Shin-Ae ) - Pusan National University School of Medicine Department of Parasitology
 ( Kim Yun-Seong ) - Pusan National University College of Medicine Department of Internal Medicine
 ( Kim Ki-Uk ) - Pusan National University College of Medicine Department of Internal Medicine
 ( Lee Min-Ki ) - Pusan National University College of Medicine Department of Internal Medicine
옥미선 ( Ock Mee-Sun ) - Kosin University College of Medicine Department of Parasitology
차희재 ( Cha Hee-Jae ) - Kosin University College of Medicine Department of Parasitology
 ( Yu Hak-Sun ) - Pusan National University School of Medicine Department of Parasitology

Abstract


We have reported that a 24 kDa protein (22U homologous; As22U) of Anisakis simplex larvae could elicit several Th2-related chemokine gene expressions in the intestinal epithelial cell line which means that As22U may play a role as an allergen. In order to determine the contribution of As22U to allergic reactions, we treated mice with 6 times intra-nasal application of recombinant As22U (rAs22U). In the group challenged with rAs22U and ovalbumin (OVA), the number of eosinophils in the bronchial alveolar lavage fluid (BALF) was significantly increased, as compared to the group receiving only OVA. In addition, mice treated with rAs22U and OVA showed significantly increased airway hyperresponsiveness. Thus, severe inflammation around the airway and immune cell recruitment was observed in mice treated with rAs22U plus OVA. The levels of IL-4, IL-5, and IL-13 cytokines in the BALF increased significantly after treatment with rAs22U and OVA. Similarly, the levels of anti-OVA specific IgE and IgG1 increased in mice treated with rAs22U and OVA, compared to those treated only with OVA. The Gro-α (CXCL1) gene expression in mouse lung epithelial cells increased instantly after treatment with rAs22U, and allergy-specific chemokines eotaxin (CCL11) and thymus-and-activation-regulated-chemokine (CCL17) gene expressions significantly increased at 6 hr after treatment. In conclusion, rAs22U may induce airway allergic inflammation, as the result of enhanced Th2 and Th17 responses.

키워드

Anisakis simplex; As22U; allergic airway inflammation; excretory secretory protein
원문 및 링크아웃 정보
  
등재저널 정보
SCI(E)
MEDLINE
KCI
KoreaMed
KAMS