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Molecular Identification of a Trichinella Isolate from a Naturally Infected Pig in Tibet, China

Korean Journal of Parasitology 2011년 49권 4호 p.381 ~ 384
 ( Li Ling Zhao ) - Zhengzhou University Medical College Department of Parasitology

 ( Wang Zhong Quan ) - Zhengzhou University Medical College Department of Parasitology
 ( Jiang Peng ) - Zhengzhou University Medical College Department of Parasitology
 ( Zhang Xi ) - Zhengzhou University Medical College Department of Parasitology
 ( Ren Hui Jun ) - Zhengzhou University Medical College Department of Parasitology
 ( Cui Jing ) - Zhengzhou University Medical College Department of Parasitology

Abstract


The first human case with trichinellosis was reported in 1964 in Tibet, China. However, up to the present, the etiological agent of trichinellosis has been unclear. The aim of this study was to identify a Tibet Trichinella isolate at a species level by PCR-based methods. Multiplex PCR revealed amplicon of the expected size (173 bp) for Trichinella spiralis in assays containing larval DNA from Tibet Trichinella isolate from a naturally infected pig. The Tibet Trichinella isolate was also identified by PCR amplification of the 5S ribosomal DNA intergenic spacer region (5S ISR) and mitochondrial large-subunit ribosomal RNA (mt-lsrDNA) gene sequences. The results showed that 2 DNA fragments (749 bp and 445 bp) of the Tibet Trichinella isolate were identical to that of the reference isolates of T. spiralis. The Tibet Trichinella isolate might be classifiable to T. spiralis. This is the first report on T. spiralis in southwestern China.

키워드

Trichinella spiralis; taxonomy; molecular identification; Tibet; China
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