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Morphologic and Genetic Identification of Taenia Tapeworms in Tanzania and DNA Genotyping of Taenia solium

Korean Journal of Parasitology 2011년 49권 4호 p.399 ~ 403
 ( Eom Kee-Seon ) - Chungbuk National University College of Medicine Department of Parasitology

 ( Chai Jong-Yil ) - Seoul National University College of Medicine Department of Parasitology and Tropical Medicine
 ( Yong Tai-Soon ) - Yonsei University College of Medicine Department of Environmental Medical Biology
 ( Min Duk-Young ) - Eulji University School of Medicine Department of Microbiology and Immunology
임한종 ( Rim Han-Jong ) - Korea University College of Medicine Department of Parasitology
 ( Kihamia Charles ) - United Republic of Tanzania Muhimbili University College of Health Sciences Department of Parasitology
 ( Jeon Hyeong-Kyu ) - Chungbuk National University College of Medicine Department of Parasitology

Abstract


Species identification of Taenia tapeworms was performed using morphologic observations and multiplex PCR and DNA sequencing of the mitochondrial cox1 gene. In 2008 and 2009, a total of 1,057 fecal samples were collected from residents of Kongwa district of Dodoma region, Tanzania, and examined microscopically for helminth eggs and proglottids. Of these, 4 Taenia egg positive cases were identified, and the eggs were subjected to DNA analysis. Several proglottids of Taenia solium were recovered from 1 of the 4 cases. This established that the species were T. solium (n=1) and T. saginata (n=3). One further T. solium specimen was found among 128 fecal samples collected from Mbulu district in Arusha, and this had an intact strobila with the scolex. Phylegenetic analysis of the mtDNA cox1 gene sequences of these 5 isolates showed that T. saginata was basal to the T. solium clade. The mitochondrial cox1 gene sequences of 3 of these Tanzanian isolates showed 99% similarity to T. saginata, and the other 2 isolates showed 100% similarity to T. solium. The present study has shown that Taenia tapeworms are endemic in Kongwa district of Tanzania, as well as in a previously identified Mbulu district. Both T. solium isolates were found to have an "African/Latin American" genotype (cox1).

키워드

Taenia solium; Taenia saginata; multiplex PCR; egg DNA; Tanzania
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