잠시만 기다려 주세요. 로딩중입니다.

Expression of Exogenous Human Hepatic Nuclear Factor-1α by a Lentiviral Vector and Its Interactions with Plasmodium falciparum Subtilisin-Like Protease 2

Korean Journal of Parasitology 2011년 49권 4호 p.431 ~ 436
 ( Liao Shunyao ) - Wonkwang University College of Medicine Department of Infection Biology

 ( Liu Yunqiang ) - Wonkwang University College of Medicine Department of Infection Biology
 ( Zheng Bing ) - Wonkwang University College of Medicine Department of Infection Biology
 ( Cho Pyo-Yun ) - Wonkwang University College of Medicine Department of Infection Biology
 ( Song Hyun-Ok ) - Wonkwang University College of Medicine Department of Infection Biology
 ( Lee Yun-Seok ) - Namseoul University Department of Health Administration
 ( Jung Suk-Yul ) - Namseoul University Department of Biomedical Laboratory Science
 ( Park Hyun ) - Wonkwang University College of Medicine Department of Infection Biology

Abstract


The onset, severity, and ultimate outcome of malaria infection are influenced by parasite-expressed virulence factors as well as by individual host responses to these determinants. In both humans and mice, liver injury follows parasite entry, persisting to the erythrocytic stage in the case of infection with the fatal strain of Plasmodium falciparum. Hepatic nuclear factor (HNF)-1α is a master regulator of not only the liver damage and adaptive responses but also diverse metabolic functions. In this study, we analyzed the expression of host HNF-1α in relation to malaria infection and evaluated its interaction with the 5’-untranslated region of subtilisin-like protease 2 (subtilase, Sub2). Recombinant human HNF-1α expressed by a lentiviral vector (LV HNF-1α) was introduced into mice. Interestingly, differences in the activity of the 5’-untranslated region of the Pf-Sub2 promoter were detected in 293T cells, and LV HNF-1α was observed to influence promoter activity, suggesting that host HNF-1α interacts with the Sub2 gene.

키워드

Plasmodium falciparum; hepatic nuclear factor-1α; subtilisin-like protease 2; lentiviral vector
원문 및 링크아웃 정보
  
등재저널 정보
SCI(E)
MEDLINE
KCI
KoreaMed
KAMS