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Two novel synthetic peptides inhibit quorum sensing-dependent biofilm formation and some virulence factors in Pseudomonas aeruginosa PAO1

Journal of Microbiology 2019년 57권 7호 p.618 ~ 625
 ( Taha Mostafa N. ) - Nahda University Faculty of Pharmacy Department of Microbiology and Immunology

 ( Saafan Amal E. ) - Menoufia University Faculty of Pharmacy Department of Pharmaceutical Microbiology
 ( Ahmedy A. ) - Cairo University Faculty of Pharmacy Department of Microbiology and Immunology
 ( El Gebaly Eman ) - Beni-Suef University Faculty of Pharmacy Department of Microbiology and Immunology
 ( Khairalla Ahmed S. ) - Beni-Suef University Faculty of Pharmacy Department of Microbiology and Immunology

Abstract


Quorum sensing (QS) regulates virulence factor expression in Pseudomonas aeruginosa. Inhibiting the QS-controlled virulence factors without inhibiting the growth of P. aeruginosa is a promising approach for overcoming the widespread resistance of P. aeruginosa. This study was proposed to investigate the effects of two novel synthetic peptides on the biofilm development and virulence factor production of P. aeruginosa. The tested strain was P. aeruginosa PAO1. The results indicated that both of the synthetic peptides (LIVRHK and LIVRRK) inhibited (P < 0.05) the formation of biofilms and the production of virulence factors, including pyocyanin, protease, and rhamnolipids, without inhibiting the growth of PAO1. Additionally, we detected transcriptional changes related to QS and found a significant reduction in the levels of gene expression of lasI, lasR, rhlI, and rhlR. This study demonstrates that LIVRRK and LIVRHK are novel synthetic peptides that can act as potent inhibitors of QS-regulated virulence factors in P. aeruginosa. Moreover, these synthetic peptides have potential applications in the treatment of biofilmrelated diseases. Both peptides may be able to control chronic infections and biofilm-associated problems of P. aeruginosa.

키워드

biofilm; peptides; quorum sensing; virulence factors
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