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BMP4-Induced Differentiation of Human Hair Follicle Neural Crest Stem Cells into Precursor Melanocytes from Hair Follicle Bulge

Annals of Dermatology 2020년 32권 5호 p.409 ~ 416
윤정호, 김호진, 김정환, 김태훈, 서창훈, 성영관, 김기호,
소속 상세정보
윤정호 ( Yoon Jung-Ho ) - Dong-A University College of Medicine Department of Dermatology
김호진 ( Kim Ho-Jin ) - Dong-A University College of Medicine Department of Dermatology
김정환 ( Kim Jung-Hwan ) - Dong-A University College of Medicine Department of Dermatology
김태훈 ( Kim Tae-Hoon ) - Dong-A University College of Medicine Department of Dermatology
서창훈 ( Seo Chang-Hoon ) - Kyungpook National University School of Medicine Department of Immunology
성영관 ( Sung Yeong-Kwan ) - Dong-A University College of Medicine Department of Dermatology
김기호 ( Kim Ki-Ho ) - Dong-A University College of Medicine Department of Dermatology

Abstract


Background: Vitiligo is a skin depigmentation disorder, for which, repigmentation treatment with combined follicular unit extraction (FUE) graft and narrowband ultraviolet B (NBUVB) is considered superior to micro-punch graft therapy. BMP4 can induce MITF expression in Neural crest stem cells (NCSCs), and α-MSH subsequently promotes the differentiation of MITF-expressing cells along the melanocyte lineage.

Objective: To investigate why FUE grafting is superior to epidermal mini grafting in promoting hair follicles (HF) melanocyte cell survival and longevity, we planned the in vitro experiments HF bulge NCSCs differentiate into melanocyte precursors under the co-treatment of BMP4 and α-MSH.

Methods: Cells that migrated from the HF bulge of scalp were cultured and assessed using immunofluorescence. Transcriptome analysis was performed on RNA sequencing results.

Results: Basic fibroblast growth factor promotes the proliferation and survival of NCSCs, with spontaneous differentiation into SOX10+/SOX2+ glial progenitors, but not into SOX10+/MITF+ precursor melanocytes. Both BMP4 and α-MSH promoted the differentiation into MITF-expressing cells. RNA sequencing revealed a downregulation in neuregulin-1 (NRG1) and sermaphorin 3C (SEMA3C), and upregulation in WNT10A. Furthermore, FUE grafting had a source of reservoir melanocytes superior to mini- grafting in treatment for vitiligo.

Conclusion: We obtained SOX10+/MITF+ precursor melanocytes through an induction of differentiation along the melanocyte lineage by BMP4 and α-MSH. According to the RNA sequencing results that NRG1 and SEMA3C were downregulated and WNT10A was upregulated, we postulated that HF NCSCs differentiated into melanocyte by co-treatment of BMP4 and α-MSH. Overall, FUE grafting is a more robust and substitutive treatment option for vitiligo.

키워드

Hair follicle; Melanocytes; Neural crest; Neuregulin-1; Stem cells

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