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Qi-activating quercetin alleviates mitochondrial dysfunction and neuroinflammation in vivo and in vitro

Archives of Pharmacal Research 2020년 43권 5호 p.553 ~ 566
강소라, Piao Ying, 강영철, Lim Su-Yeol, Pak Young-Mi Kim,
소속 상세정보
강소라 ( Kang So-Ra ) - Kyung Hee University Graduate School Department of Neuroscience
 ( Piao Ying ) - Kyung Hee University Graduate School Department of Neuroscience
강영철 ( Kang Young-Cheol ) - Kyung Hee University Graduate School Department of Neuroscience
 ( Lim Su-Yeol ) - Kyung Hee University Graduate School Department of Biomedical Science
 ( Pak Young-Mi Kim ) - Kyung Hee University College of Medicine Department of Physiology

Abstract


Parkinson's disease (PD) is a multifactorial neurodegenerative disease manifesting mitochondrial damages and neuroinflammation. Qi is defined as a natural power that can regulate the energy flow in Oriental medicine, whereas mitochondria generate energy power in Western medicine. We investigated whether Qi-enhancing component in Oriental herb medicines could activate mitochondrial activities. Quercetin was found as a major bioactive compound in most Qi-activating Oriental herb medicines through online search for active compounds in several Oriental Medicine databases. We then investigated if quercetin could reverse 1-methyl-4-phenylpyridinium (MPP+)-induced mitochondrial dysfunction and lipopolysaccharide (LPS)-induced neuroinflammation. Mitochondrial activities were monitored based on complex 1 NADH dehydrogenase activities, ATP contents, mitochondrial membrane potential, cellular/mitochondrial reactive oxygen species, and oxygen consumption rate in SH-SY5Y cells. Quercetin at concentration up to 20 μg/ml was not cytotoxic to SH-SY5Y cells. Pre-treatment with quercetin significantly protected mitochondrial damages in 1 mM MPP+- or 100 ng/ml LPS-treated cells. Quercetin increased expression levels of tyrosine hydroxylase and mitochondria controlling proteins. When in vivo effects of quercetin were assessed by immunohistochemical staining of tissue sections from LPS-injected mice brains, quercetin reduced the activation of microglia and astrocytes in the hippocampus and substantia nigra of LPS-injected mice. Our data suggest that Qi-activating quercetin might be therapeutically effective for neuroinflammation-mediated neurodegeneration by alleviating mitochondrial damages.

키워드

Mitochondria; Herbal medicine; Qi-activation; Neuroinflammation; Parkinson’s disease

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