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Objective Verification of Acute Tinnitus and Validation of Efficacy of Systemic Steroids in Rats

Journal of Korean Medical Science 2020년 35권 13호 p.81 ~ 81
Kim Yeong-Cheol, 김희진, Kim Young-Seon, Jung Seo-Kyung, 박일용, 정연훈, 장정훈,
소속 상세정보
 ( Kim Yeong-Cheol ) - Ajou University School of Medicine Department of Otolaryngology
김희진 ( Kim Hee-Jin ) - Hallym University Dongtan Sacred Heart Hospital Department of Otorhinolaryngology-Head and Neck Surgery
 ( Kim Young-Seon ) - Ajou University School of Medicine Department of Otolaryngology
 ( Jung Seo-Kyung ) - Ajou University School of Medicine Department of Otolaryngology
박일용 ( Park Il-Yong ) - Dankook University College of Medicine Department of Biomedical Engineering
정연훈 ( Choung Yun-Hoon ) - Ajou University School of Medicine Department of Otolaryngology
장정훈 ( Jang Jeong-Hun ) - Ajou University School of Medicine Department of Otolaryngology

Abstract


Background: This study was performed to identify acute tinnitus and evaluate the efficacy of steroids for noise-induced acute tinnitus by measuring the gap-prepulse inhibition of the acoustic startle (GPIAS) value in an animal model.

Methods: Nineteen rats (the noise group [n = 7] and the noise + dexamethasone [DEX] group [n = 12]) were exposed to narrow-band noise centered at 16 kHz from a sound generator for 4 hours. The noise + DEX group received intraperitoneal steroid administration daily for 5 days (1.5 mg/kg/day) after completing noise exposure. Auditory brainstem response and GPIAS value were measured just prior to, and 1 day after noise exposure and on days 1 and 10 days after completing steroid administration. The changes in cochlear structure were evaluated by histological analysis.

Results: The threshold shift was checked 1 and 10 days after intraperitoneal steroid injection, and no differences in threshold shift were observed between the two groups in each frequency except for 32 kHz 1 day after steroid injection. The mean GPIAS value in the noise + DEX group (36.4% ± 14.1%) was significantly higher than that in the noise group (16.4% ± 18.8%) 10 days after intraperitoneal steroid administration (P = 0.017). There were no pathological changes associated with noise trauma in the two groups as determined on hematoxylin and eosin and immunohistochemical staining.

Conclusion: An acute tinnitus model with minimal structural changes by noise exposure was set up, and used to verify tinnitus objectively by measuring the GPIAS value. Steroid therapy for control of tinnitus was validated in this animal model.

키워드

Tinnitus; GPIAS; Auditory Brainstem Response; Dexamethasone; Noise-Induced Hearing Loss

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