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NUP210 and MicroRNA-22 Modulate Fas to Elicit HeLa Cell Cycle Arrest

Yonsei Medical Journal 2020년 61권 5호 p.371 ~ 381
 ( Gu Qiao ) - Soochow University Third Affiliated Hospital Department of Gynecology and Obstetrics

 ( Hou Wenjie ) - Soochow University First Affiliated Hospital Department of Gynecology and Obstetrics
 ( Liu Huan ) - Changzhou TCM Hospital Department of Pathology
 ( Shi Lijuan ) - Soochow University Third Affiliated Hospital Department of Gynecology and Obstetrics
 ( Zhu Zonghao ) - Soochow University Third Affiliated Hospital Department of Gynecology and Obstetrics
 ( Ye Wenfeng ) - Soochow University Third Affiliated Hospital Department of Gynecology and Obstetrics
 ( Ni Xiaoyuan ) - Tongxiang DiYi Renming Hospital Department of General Surgery

Abstract


Purpose: Cervical cancer is one of the most fatal diseases among women in under-developed countries. To improve cervical cancer treatment, discovery of new targets is needed. In this study, we investigated the expression of NUP210, miR-22, and Fas in cervical cancer tissues and their functions in cell cycle regulation.

Materials and Methods: We detected and compared the expression levels of NUP210, miR-22, and Fas in cervical cancer tissues with paired normal tissues using immunohistochemistry, Western blot, and real-time quantitative polymerase chain reaction. NUP210 was knocked down in HeLa cells via lentivirus, followed by cell cycle and proliferation analysis. Using a luciferase reporter assay, we explored the link between miR-22 and NUP210. We overexpressed miR-22 in HeLa cells and analyzed cell cycle and proliferation function. We then overexpressed miR-22 in NUP210 knockdown cells to explore the connection between Fas and miR-22-NUP210 signaling.

Results: We found that NUP210 was overexpressed in cervical cancer patients. Knocking down NUP210 restored cell apoptosis and proliferation. We confirmed miR-22 as a regulator of NUP210 and verified that miR-22 was inhibited in cervical cancer development. We also found that restoring miR-22 expression could induce cell apoptosis. Finally, we found that miR-22-regulated expression of NUP210 could alter Fas expression and, in turn, elicit cell cycle arrest and proliferation.

Conclusion: miR-22 in cervical cancer is downregulated, resulting in NUP210 overexpression and inhibition of Fas-induced cell apoptosis.

키워드

Cervical cancer; apoptosis; cell cycle arrest; Fas; miR-22; NUP210
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