A cohort study on the association of MDM2 SNP309 with lung cancer risk in Bangladeshi population
Al Reza Hasan, Anamika Wardatul Jannat, Chowdhury Md. Miraj Kobad, Mostafa Mohammad Golam, Uddin M. Aftab,
소속 상세정보
( Al Reza Hasan ) - University of Dhaka Department of Genetic Engineering and Biotechnology
( Anamika Wardatul Jannat ) - University of Dhaka Department of Genetic Engineering and Biotechnology
( Chowdhury Md. Miraj Kobad ) - University of Dhaka Department of Genetic Engineering and Biotechnology
( Mostafa Mohammad Golam ) - National Institute of Cancer Research and Hospital Department of Histopathology
( Uddin M. Aftab ) - University of Dhaka Department of Genetic Engineering and Biotechnology
Abstract
Background/Aims: Bangladesh is a densely populated country with an increased incidence of lung cancer, mostly due to smoking. Therefore, elucidating the association of mouse double minute 2 homolog (MDM2) single nucleotide polymorphism (SNP) 309 (rs2279744) with lung cancer risk from smoking in Bangladeshi population has become necessary.
Methods: DNA was extracted from blood samples of 126 lung cancer patient and 133 healthy controls. The MDM2 SNP309 was genotyped by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP), using the restriction enzymes MspA1I. Logistic regression was then carried out to calculate odds ratios (ORs) and 95% confidence intervals (CIs) to estimate the risk of lung cancer. A meta-analysis of SNP309 was also carried out on 12,758 control subjects and 11,638 patient subjects.
Results: In multivariate logistic regression, significantly increased risk of lung cancer was observed for MDM2 SNP309 in the dominant model (TG + GG vs. TT: OR, 2.13; 95% CI, 1.29 to 3.53). Stratification analysis revealed that age, sex, obesity, and smoking also increases the risk of lung cancer when carrying the MDM2 SNP309. Our meta-analysis revealed that MDM2 SNP309 was considerably associated with lung cancer in Asian populations (TG + GG vs. TT: OR, 1.32; 95% CI , 1.12 to 1.56; p = 0.019 for heterogeneity).
Conclusions: The MDM2 SNP309 was associated with high risk of lung cancer in Bangladeshi and Asian population, particularly with increased age, smoking, and body mass index.
키워드
Lung neoplasms ; MDM2 gene ; MDM2 SNP309 ; Meta-analysis
원문 및 링크아웃 정보
등재저널 정보