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A cohort study on the association of MDM2 SNP309 with lung cancer risk in Bangladeshi population

Korean Journal of Internal Medicine 2020년 35권 3호 p.672 ~ 681
 ( Al Reza Hasan ) - University of Dhaka Department of Genetic Engineering and Biotechnology

 ( Anamika Wardatul Jannat ) - University of Dhaka Department of Genetic Engineering and Biotechnology
 ( Chowdhury Md. Miraj Kobad ) - University of Dhaka Department of Genetic Engineering and Biotechnology
 ( Mostafa Mohammad Golam ) - National Institute of Cancer Research and Hospital Department of Histopathology
 ( Uddin M. Aftab ) - University of Dhaka Department of Genetic Engineering and Biotechnology

Abstract


Background/Aims: Bangladesh is a densely populated country with an increased incidence of lung cancer, mostly due to smoking. Therefore, elucidating the association of mouse double minute 2 homolog (MDM2) single nucleotide polymorphism (SNP) 309 (rs2279744) with lung cancer risk from smoking in Bangladeshi population has become necessary.

Methods: DNA was extracted from blood samples of 126 lung cancer patient and 133 healthy controls. The MDM2 SNP309 was genotyped by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP), using the restriction enzymes MspA1I. Logistic regression was then carried out to calculate odds ratios (ORs) and 95% confidence intervals (CIs) to estimate the risk of lung cancer. A meta-analysis of SNP309 was also carried out on 12,758 control subjects and 11,638 patient subjects.

Results: In multivariate logistic regression, significantly increased risk of lung cancer was observed for MDM2 SNP309 in the dominant model (TG + GG vs. TT: OR, 2.13; 95% CI, 1.29 to 3.53). Stratification analysis revealed that age, sex, obesity, and smoking also increases the risk of lung cancer when carrying the MDM2 SNP309. Our meta-analysis revealed that MDM2 SNP309 was considerably associated with lung cancer in Asian populations (TG + GG vs. TT: OR, 1.32; 95% CI , 1.12 to 1.56; p = 0.019 for heterogeneity).

Conclusions: The MDM2 SNP309 was associated with high risk of lung cancer in Bangladeshi and Asian population, particularly with increased age, smoking, and body mass index.

키워드

Lung neoplasms ; MDM2 gene ; MDM2 SNP309 ; Meta-analysis
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