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Metabolomes and transcriptomes revealed the saponin distribution in root tissues of Panax quinquefolius and Panax notoginseng

Journal of Ginseng Research 2020년 44권 6호 p.757 ~ 769
Wei Guangfei, Yang Feng, Wei Fugang, Zhang Lianjuan, Gao Ying, Qian Jun, Chen Zhongjian, Jia Zhengwei, Wang Yong, Su He, Dong Linlin, Xu Jiang, Chen Shilin,
소속 상세정보
 ( Wei Guangfei ) - China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica
 ( Yang Feng ) - China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica
 ( Wei Fugang ) - Wenshan Miaoxiang Notoginseng Technology Co. Ltd.
 ( Zhang Lianjuan ) - China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica
 ( Gao Ying ) - Chinese Academy of Forestry Institute of Desertification Studies
 ( Qian Jun ) - China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica
 ( Chen Zhongjian ) - Wenshan University Institute of Sanqi Research
 ( Jia Zhengwei ) - Waters Corporation Shanghai Science and Technology Co. Ltd.
 ( Wang Yong ) - Wenshan University Institute of Sanqi Research
 ( Su He ) - China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica
 ( Dong Linlin ) - China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica
 ( Xu Jiang ) - China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica
 ( Chen Shilin ) - China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica

Abstract


Background: Panax quinquefolius and Panax notoginseng are widely used and well known for their pharmacological effects. As main pharmacological components, saponins have different distribution patterns in the root tissues of Panax plants.

Methods: In this study, the representative ginsenosides were detected and quantified by desorption electrospray ionization mass spectrometry and high-performance liquid chromatography analysis to demonstrate saponin distribution in the root tissues of P. quinquefolius and P. notoginseng, and saponin metabolite profiles were analyzed by metabolomes to obtain the biomarkers of different root tissues. Finally, the transcriptome analysis was performed to demonstrate the molecular mechanisms of saponin distribution by gene profiles.

Results: There was saponin distribution in the root tissues differed between P. quinquefolius and P. notoginseng. Eight-eight and 24 potential biomarkers were detected by metabolome analysis, and a total of 340 and 122 transcripts involved in saponin synthesis that were positively correlated with the saponin contents (R > 0.6, P < 0.05) in the root tissues of P. quinquefolius and P. notoginseng, respectively. Among them, GDPS1, CYP51, CYP64, and UGT11 were significantly correlated with the contents of Rg1, Re, Rc, Rb2, and Rd in P. quinquefolius. UGT255 was markedly related to the content of R1; CYP74, CYP89, CYP100, CYP103, CYP109, and UGT190 were markedly correlated with the Rd content in P. notoginseng.

Conclusions: These results provided the visual and quantitative profiles of and confirmed the pivotal transcripts of CYPs and UGTs regulating the saponin distribution in the root tissues of P. quinquefolius and P. notoginseng.

키워드

Metabolome; Panax plants; Root tissues; Saponin distribution; Transcriptome

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