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Effect of BMS-470539 on lipopolysaccharide-induced neutrophil activation

대한마취과학회지 2020년 73권 2호 p.151 ~ 157
이성헌 ( Lee Seong-Heon ) - Chonnam National University Medical School and Hospital Department of Anesthesiology and Pain Medicine

 ( Ju Wan ) - Chonnam National University Medical School and Hospital Department of Anesthesiology and Pain Medicine
 ( Tin Tran Duc ) - Chonnam National University Center for Creative Biomedical Scientists
김정민 ( Kim Joung-Min ) - Chonnam National University Medical School and Hospital Department of Anesthesiology and Pain Medicine
이정석 ( Lee Jeong-Seok ) - Gwangju Christian Hospital Department of Anesthesiology and Pain Medicine
박천희 ( Park Cheon-Hee ) - Gwangju Christian Hospital Department of Anesthesiology and Pain Medicine
곽상현 ( Kwak Sang-Hyun ) - Chonnam National University Medical School and Hospital Department of Anesthesiology and Pain Medicine

Abstract


Background: BMS-470539, a recently introduced selective agonist of the melanocortin 1 receptor, is known to have anti-inflammatory properties. In this study, we investigated the effects of BMS-470539 on lipopolysaccharide (LPS)-induced inflammatory responses and delayed apoptosis with its signaling pathways in human neutrophils.

Methods: Isolated human neutrophils were incubated with various concentrations of BMS-470539 (1, 10, and 100 μM) in the presence or absence of LPS (100 ng/ml), and the expression of pro-inflammatory cytokines, such as tumor necrosis factor alpha, interleukin (IL)-6, and IL-1β, were assessed. The effects of BMS-470539 on the expression of mitogen-activated protein kinases (MAPKs), such as p38, extracellular-signal-regulated kinase 1/2, and c-Jun N-terminal kinase, and the expression of nuclear factor kappa B (NF-κB) in LPS-stimulated human neutrophils, were evaluated by enzyme-linked immunosorbent assay. Neutrophil apoptosis was also measured by fluorescence-activated cell sorting (annexin V/propidium iodide) in LPS-stimulated neutrophils under treatment with BMS-470539.

Results: BMS-470539 attenuated LPS-induced expression of pro-inflammatory cytokines, and phosphorylation of MAPKs and NF-κB. LPS stimulation reduced neutrophil apoptosis compared to the controls; however, BMS-470539 significantly inhibited the reduction of neutrophil apoptosis.

Conclusions: BMS-470539 can suppress the inflammatory responses of LPS-stimulated neutrophils by inhibition of MAPK pathways or NF-κB pathway, and it can also inhibit LPS-delayed neutrophil apoptosis.

키워드

Apoptosis; BMS-470539; Cytokines; Lipopolysaccharides; Mitogen-activated protein kinases; Neutrophils; nuclear factor-kappa B
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