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Effect of Zhongyi paste on inflammatory pain in mice by regulation of the extracellular regulated protein kinases 1/2?cyclooxygenase-2?prostaglandin E2 pathway

Korean Journal of Pain 2020년 33권 4호 p.335 ~ 343
Xiao Ailan, Wu Chuncao, Kuang Lei, Lu Weizhong, Zhao Xin, Kuang Zhiping, Hao Na,
소속 상세정보
 ( Xiao Ailan ) - Chongqing Traditional Chinese Medicine Hospital Department of Orthopaedic Surgery
 ( Wu Chuncao ) - Chongqing Traditional Chinese Medicine Hospital Office of Academic Research
 ( Kuang Lei ) - Chongqing Traditional Chinese Medicine Hospital Department of Orthopaedic Surgery
 ( Lu Weizhong ) - Chongqing Traditional Chinese Medicine Hospital Department of Orthopaedic Surgery
 ( Zhao Xin ) - Chongqing University of Education Chongqing Collaborative Innovation Center for Functional Food
 ( Kuang Zhiping ) - Chongqing Traditional Chinese Medicine Hospital Department of Orthopaedic Surgery
 ( Hao Na ) - Chongqing Traditional Chinese Medicine Hospital Department of Orthopaedic Surgery

Abstract


Background: Zhongyi paste is a traditional Chinese medicine herbal paste that is externally applied to reduce inflammation and relieve pain.

Methods: An acute foot swelling inflammation model in C57BL/6J mice was established by carrageenan-induced pathogenesis. Zhongyi paste raised the pain threshold and also reduced the degree of swelling in mice with carrageenan-induced foot swelling.

Results: Analysis indicated that serum tumor necrosis factor-alpha, interleukin-1 beta, and prostaglandin E2 (PGE2) cytokine levels and PGE2 levels in the paw tissue of the mice were decreased by Zhongyi paste treatment. The quantitative polymerase chain reaction and western blot results showed that Zhongyi paste downregulated the mRNA and protein expression of extracellular signal-regulated kinase 1/2 (ERK1/2), and cyclooxygenase-2 (COX-2), and also downregulated the mRNA expression of PGE2. At the same time, the Zhongyi paste exerted a stronger effect as an external drug than that of indomethacin, which is an oral drug, and voltaren, which is an externally applied drug.

Conclusions: Our results indicated that Zhongyi paste is a very effective drug to reduce inflammatory swelling of the foot, and its mechanism of action is related to regulation of the ERK1/2?COX-2?PGE2 pathway.

키워드

Biochemistry; Cyclooxygenase 2; Herbal Medicine; Inflammation; Medicine; Chinese Traditional; Mitogen-Activated Protein Kinase 3; Pain; RNA; Messenger

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