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MIA로 유발된 골관절염 동물모델에서 산수유 추출물의 골관절염 개선 효과

Protective Effect of Corni Fructus Extracts on MIA-induced Animal Model of Osteoarthritis: Effect of Corni Fructus Extracts on OA

대한한방내과학회지 2020년 41권 1호 p.1 ~ 13
백경민, 안유민, 신미래, 김민주, 이진아, 노성수,
소속 상세정보
백경민 ( Baek Kyung-Min ) - Daegu Haany University Daegu Oriental Hospital Department of Cardiovascular and Neurologic Diseases of Korean Internal Medicine
안유민 ( An Yu-Min ) - Daegu Haany University Daegu Oriental Hospital Department of Cardiovascular and Neurologic Diseases of Korean Internal Medicine
신미래 ( Shin Mi-Rae ) - Daegu Haany University College of Korean Medicine Department of Herbology
김민주 ( Kim Min-Ju ) - Daegu Haany University College of Korean Medicine Department of Herbology
이진아 ( Lee Jin-A ) - Daegu Haany University College of Korean Medicine Department of Herbology
노성수 ( Roh Seong-Soo ) - Daegu Haany University College of Korean Medicine Department of Herbology

Abstract


Objectives: Osteoarthritis (OA) is a chronic and degenerative joint disease characterized by progressive degeneration of articular cartilage. Inflammation is a recognized and important factor of OA progression. The present study was designed to investigate the protective effect of Corni Fructus water extract (CFW) on a monosodium iodoacetate (MIA)-induced rat model of OA.

Methods: Osteoarthritis was induced by injection of MIA (50 μL; 80 mg/mL) into the knee joint cavity of rats. After an adaptation period for seven days, the rats were divided into 4 groups (n=8/group): normal, control, indomethacin-treated (5 mg/kg), and CFW-treated (200 mg/kg) groups. The rats were treated orally for 14 days. Pain was evaluated by determining hind paw weight distribution. For biochemical analyses, we measured the changes in reactive oxygen species (ROS) and peroxynitrite (ONOO-) in the knee joint. The presence of anti-oxidant proteins and inflammatory proteins was determined by western blotting.

Results: The administration of CFW significantly improved the hind paw weight distribution. The ROS and ONOO- levels of knee joint were significantly decreased in the CFW group. CFW inhibited the production of inflammatory mediators, such as COX-2, and inflammatory cytokines, including IL-6 and IL-1β, via the NF-κB signaling pathway. The expression of anti-oxidant enzymes, such as catalase and GPx-1/2 also increased significantly.

Conclusions: The findings indicate that CFW has a therapeutic and protective effect on OA by suppression of inflammation. Therefore, CFW could represent a potential and effective candidate for OA treatment.

키워드

monosodium iodoacetate; osteoarthritis; Corni Fructus; inflammation; NF-κBp65

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