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근육세포주에서 당 흡수 및 대사 조절에 대한 황기탕 에탄올 추출물의 효과

Effects of a Hwanggi-tang Ethanol Extract on Glucose Uptake and Metabolism in Murine Myotubes

대한한방내과학회지 2020년 41권 4호 p.599 ~ 611
장철용, 신선호, 신용진,
소속 상세정보
장철용 ( Jang Chul-Yong ) - Wonkwang University College of Korean Medicine Department of Internal Medicine
신선호 ( Shin Sun-Ho ) - Wonkwang University College of Korean Medicine Department of Internal Medicine
신용진 ( Shin Yong-Jeen ) - Wonkwang University College of Korean Medicine Department of Internal Medicine

Abstract


Objectives: The aim of this study was to evaluate the effects of Hwanggi-tang on glucose digestion, uptake, and metabolism in murine C2C12 myotubes.

Methods: Hwanggi-tang was prepared according to the Dong-ui-bo-gam (≪東醫寶鑑≫) prescription by 70% ethanol extraction. The effect on glucose digestion was examined by determining the inhibitory effect of Hwanggi-tang on α-glucosidase activity. We also compared and verified the gene and protein expression of genes related to glucose uptake in C2C12 myotubes treated with Hwanggi-tang or insulin. Glucose metabolism was assessed by the expression levels of associated enzymes.

Results: Hwanggi-tang caused a dose-dependent inhibition of α-glucosidase activity, induced glucose uptake by activation of the PI3K/Akt/mTOR pathway in the insulin signaling pathway, and promoted glucose oxidation and β-oxidation.

Conclusions: Hwanggi-tang exerts an anti-diabetic effect on murine myotubes by inhibiting glucose digestion and inducing glucose uptake and consumption.

키워드

carbohydrate metabolism; diabetes mellitus; herbal medicine; skeletal muscle fiber

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