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갈근황연황금탕(葛根黃蓮黃芩湯)과 소풍활혈탕(疎風活血湯)이 HUVEC 내에 MCP-1, ICAM-1, VCAM-1 and eNOS의 유전자 발현량에 대해 미치는 영향

The Effect of Galkunhwanglyeonhwanggum-tang (GGT) and Sopunghwalhyeol-tang (SPT) on Gene Expression Levels of MCP-1, ICAM-1, VCAM-1, and eNOS in HUVECs

대한한방내과학회지 2020년 41권 4호 p.668 ~ 675
정현진, 전상윤, 장혜연, 김민욱,
소속 상세정보
정현진 ( Jeong Hyun-Jin ) - Gwangju Dong-Shin University Oriental Hosipital Department of Internal Korean Medicine
전상윤 ( Jeon Sang-Yoon ) - Mokpo Dong-Shin University Oriental Hosipital Department of Internal Korean Medicine
장혜연 ( Jang Hye-Yeon ) - Gwangju Dong-Shin University Oriental Hosipital Department of Internal Korean Medicine
김민욱 ( Kim Min-Wook ) - Gwangju Dong-Shin University Oriental Hosipital Department of Acupuncture and Moxibustion Medicine

Abstract


Objectives: The aim of this study was to compare the effects of Galkunhwanglyeonhwanggum-tang (GGT), and Sopunghwalhyeol-tang (SPT) on gene expression of MCP-1, ICAM-1, VCAM-1, and eNOS in human umbilical vein endothelial cells (HUVECs).

Methods: HUVECs were treated with GGT and SPT at concentrations of 50, 100, and 200 μg/mL. Gene expression of MCP-1, ICAM-1 ,VCAM-1, and eNOS in HUVECs was analyzed by the polymerase chain reaction (PCR), and electrophoresis was performed to verify the gene expression level.

Results: 1. MCP-1 gene expression was more strongly decreased by SPT than by GGT. 2. ICAM-1 and VCAM-1 gene expressions were more strongly decreased by SPT than by GGT 3. GGT significantly increased eNOS gene expression, but SPT did not.

Conclusions: These findings suggest that GGT and SPT regulate gene expression related to anti-inflammatory effects in HUVECs. Clinical application of these Korean medicines to diseases related to dyslipidemia, such as cardiovascular disease, will require additional in vivo experiments to verify the anti-inflammatory effects of GGT and SPT.

키워드

Galkunhwanglyeonhwanggum-tang; Sopunghwalhyeol-tang; anti-inflammatory effect; HUVEC

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