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Rhus Verniciflua Stokes Extract Suppresses Expression of Metalloproteinases, iNOS and COX-2 in THP-1 Cells Via Inhibiting NF-κB and MAPK Phosphorylation

대한한의학회지 2020년 41권 4호 p.12 ~ 26
고환주, 장은경, 김영철,
소속 상세정보
고환주 ( Ko Hwan-Joo ) - Kyung Hee University Graduate School Department of Clinical Korean Medicine
장은경 ( Jang Eun-Gyeong ) - Kyung Hee University Korea Medicine Hospital Department of Hepato-hemopoietic System
김영철 ( Kim Young-Chul ) - Kyung Hee University Korea Medicine Hospital Department of Hepato-hemopoietic System

Abstract


Objectives: The aim of this study is to investigate the mechanisms involved in the anti-inflammatory and anti-tumor effects of Rhus verniciflua Stokes (RVS) on PMA-differentiated human monocytic leukemia THP-1 cells.

Methods: Cells were treated with various concentrations of RVS decoction (0-300μg/ml) for 24, 48, and 72h. Cell viability was evaluated by MTS/PMS assay. The expressions of MMP-2, MMP-9, TIMP-1, TIMP-2, iNOS and COX-2 mRNA and proteins were measured using RT-PCR and western blotting, respectively.

Results: RVS suppressed expression of MMP-2 and MMP-9 mRNA. It also down-regulated iNOS and COX-2 mRNA and protein expression. RVS inhibited NF-κB p65 activity and the phosphorylation of Akt and MAPK (ERK and p38 MAPK). Instead, the phosphorylation of JNK is increased at a very low concentration but decreased at higher concentrations.

Conclusion: RVS is regarded to inhibit the expression of MMP and TIMP as well as iNOS and COX-2 gene expression via directly inhibiting the activation of NF-κB and phosphorylation of MAPK pathway in THP-1 cells. This suggests RVS have potential to be used as a therapeutic agent for acute myeloid leukemia (AML).

키워드

Rhus verniciflua Stokes; MMP; iNOS; COX-2; NF-κB; MAPK

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