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Immunostimulatory effects of the Pueraria lobata flower extract via MAPK signaling in RAW264.7 cells

대한한의학회지 2020년 41권 4호 p.52 ~ 63
김기태,
소속 상세정보
김기태 ( Kim Ki-Tae ) - Semyung University Department of Korean Medicine

Abstract


Objectives: In this study, we investigated the effects of Pueraria lobata Ohwi flower extracts (PLFE) on macrophages and their underlying mechanism(s) of action. PLFE increased the production of NO and cytokines (IL-6 and TNF-α) in a dose-dependent manner, indicating its immunostimulatory property. Furthermore, PLFE upregulated iNOS, COX-2, and mitogen-activated protein kinase (MAPK) signaling in RAW264.7 cells. Additionally, PLFE enhanced the phosphorylation of IκBα and subsequent IκBα degradation, thereby enabling the nuclear translocation of NF-κB. Taken together, these findings demonstrate that the immunostimulatory effects of PLFE are mediated by the nuclear translocation of the p65 subunit of NF-κB and subsequent secretion of cytokines (IL-6 and TNF-α), upregulation of iNOS and COX-2, and stimulation of MAPK signaling (JNK, ERK, and p38). Thus, PLFE may be a potential immunostimulatory therapeutic.

키워드

Pueraria lobata Ohwi flower; PLFE; immunosimulatory effect

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