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Expression of Chicken Cystatin for Improving Insect Resistance in Rice

한국식물학회지 2001년 44권 4호 p.205 ~ 212
 ( Ryu Sun-Hyo ) - Chosun University Department of Genetic Engineering

정기철 ( Chung Ki-Chul ) - Chonnam University Department of Genetic Engineering
 ( Chi Youn-Tae ) - Chonnam University Department of Genetic Engineering
정현숙 ( Cheong Hyeon-Sook ) - Chosun University Department of Genetic Engineering

Abstract


To become mature and infectious, many viruses and insects require proteolytic cleavage, which can be specifically inhibited by proteinase inhibitors. Oryzacystatin (OC), the first-described cystatin originating from rice seed, consists of two molecular species, OC-I and OC-II, both of which have antiviral activity. These intrinsic rice cystatins show a narrow inhibition spectrum and ordinarily are present in rice seeds at insufficient levels for inhibiting the cysteine proteinases of rice insect pests. In addition, our comparison of inhibitory activity (Ki value) showed that chicken cystatin (Ki 5 × 10-12 M) was more powerful than other cystatins, such as OC-I (Ki 3.02 × 10-8 M) and OC-II (l(i 0.83 × 10-8 M). Chicken cystatin also possesses a wide inhibitory spectrum against various cysteine proteinases. Here, we introduced the insecticidal chicken cystatin 8ene into rice plants to improve their insect resistance. Four highly expressive, independent transgenic lines were identified. Molecular analyses revealed that the transferred 8ene was expressed stably in the independent transgenic lines. Therefore, introducing the insecticidal cysteine proteinase inhibitor 8ene into rice plants can be part of a general development strategy for pest control.

키워드

chicken cystatin; insect resistance; rice
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