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Possible involvement of Ca2+-dependent protein kinases in spore germination of the fernOsmunda Japonica

한국식물학회지 2004년 47권 1호 p.27 ~ 32
 ( Kamachi Hiroyuki ) - Toyama University Faculty of Science Department of Environmental Biology and Chemistry

 ( Noguchi Munenori ) - Toyama University Faculty of Science Department of Environmental Biology and Chemistry
 ( Inoue Hiroshi ) - Toyama University Faculty of Science Department of Environmental Biology and Chemistry

Abstract


Fern gametophyte is a good model system to investigate signal transduction in plant cells. In this work, we examined whether CDPKs are involved in the mechanisms of spore germination of the fernOsmunda japonica. A protein extract from the spores included four CDPK isoforms with relative molecular weights of 56, 53, 49, and 47 kDa, as detected by immunoblot analysis, and they showed CDPK-like activities, as detected by in-gel protein-kinase assay. It was also found that the inhibitors effective on CDPKs, such as a general protein kinase inhibitor, K252a, and a calmodulin antagonist, W-7, largely suppressed the spore germination, and that many proteins of the spores were phosphorylated in vivo in a calcium dependent manner in the period when the spores require external Ca2+ for the germination. Furthermore, we showed that Sr2+ and Mn2+, which could substitute for Ca2+ in the spore germination, were also able to activate theOsmunda CDPKs. From these results, we concluded that CDPKs would participate in the spore germination ofO. japonica.

키워드

CDPK; fern gametophyte; Osmunda japonica; spore germination
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