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Cloning and expression analysis of 2-on-2 hemoglobin from soybean

한국식물학회지 2004년 47권 2호 p.92 ~ 98
이형석 ( Lee Hyoung-Seok ) - Seoul National University School of Biological Sciences

 ( Kim Hyo-Jeong ) - Seoul National University School of Biological Sciences
안정선 ( An Chung-Sun ) - Seoul National University School of Biological Sciences

Abstract


Hemoglobins (Hbs) are heme proteins found in all five kingdoms of living organisms. In plants, three different classes of Hbs have been identified-nonsymbiotic Hbs from diverse species, symbiotic Hbs from nitrogen-fixing plants, and so-called 2-on-2 Hbs. Here, we report the cloning and expression analysis of the 2-on-2 Hb gene,GmGLB3, from soybean. TheGmGLB3 cDNA clone encodes a protein for 172 amino acid residues. Its deduced amino acid sequence shows the highest identity (74%) with 2-on-2 Hb fromMedicago truncatula. Multiple sequence alignment confirms the conserved and signature amino acid residues previously reported with plant 2-on-2 Hbs. Genomic Southern hybridization demonstrates thatGmGLB3 has two copies in the soybean genome. Based on our northern hybridization, theGmGLB3 gene is specifically expressed in root nodules, with levels increasing in the late stage during nodule development. Its transcript level is also increased under flooding and kinetin treatments in the roots, or under flooding and 2-iP treatments in the stems. However, no transcript is detected in the leaves regardless of treatment. Therefore, we propose that theGmGLB3 gene is specifically expressed in root nodules and that its expression in other plant organs is regulated by cytokinin and/or flooding.

키워드

2-on-2 hemoglobin; cytokinin; flooding; root nodule; soybean
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