잠시만 기다려 주세요. 로딩중입니다.

Changes in antioxidant enzyme activities in detached leaves of cucumber exposed to chilling

한국식물학회지 2004년 47권 2호 p.117 ~ 123
 ( Lee Min-Ah ) - Dong-Eui University Department of Molecular Biology

전현식 ( Chun Hyun-Sik ) - Jinju National University Department of Crop Science and Biotechnology
 ( Kim Jin-Woo ) - Jinju National University Department of Crop Science and Biotechnology
 ( Lee Hyub ) - Jinju National University Department of Crop Science and Biotechnology
이동회 ( Lee Dong-Hee ) - Genomine Inc.
이진범 ( Lee Chin-Bum ) - Dong-Eui University Department of Molecular Biology

Abstract


We studied changes in biochemical and physiological status, level of oxidative damage, and antioxidant enzyme activities in detached leaves of cucumber plants (Cucumis sativus L. cv. Pyunggangnaebyungsamchuk) that were exposed to a low temperature (4°C). Chlorophyll fluorescence (Fv/Fm) declined during the chilling treatment, but was slowly restored after the tissues were returned to 25°C. Likewise, the fluorescence quenching coefficient and relative water content decreased during the stress period, but then increased during recovery. In contrast, we detected a significant rise in protein and hydrogen peroxide contents in the chilled leaves, as well as higher activities for superoxide dismutase, ascorbate peroxidase, peroxidase, and glutathione reductase. However, the level of catalase decreased not only during chilling but also after 24 h of recovery. These results indicate that exposure to low temperatures acts as an oxidative stress. Moreover, we propose that a regulating mechanism exists in the detached cucumber leaves and contains an antioxidant defense system that induces active oxygen species, thereby alleviating the effects of chilling stress within 12 h.

키워드

antioxidant enzyme; chilling stress; chlorophyll fluorescence; Cucumis sativus; H2O2
원문 및 링크아웃 정보
 
등재저널 정보