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Characterization of a RAPD fragment unique to species with hairy fruit skin in the genusActinidia

한국식물학회지 2004년 47권 3호 p.210 ~ 215
김성철 ( Kim Seong-Cheol ) - Rural Development Administration National Institute of Subtropical Agriculture

정용환 ( Jung Yong-Hwan ) - Rural Development Administration National Institute of Subtropical Agriculture
 ( Kim Mi-Sun ) - Rural Development Administration National Institute of Subtropical Agriculture
 ( Koh Seok-Chan ) - Jeju National University College of Natural Sciences Department of Life Science
 ( Song Kwan-Jeong ) - Jeju National University Faculty of Horticulture and Life Science
김호방 ( Kim Ho-Bang ) - Seoul National University School of Biological Sciences

Abstract


To develop a SCAR primer related to the hairy-fruit trait in the genusActinidia, we took a PCR-RAPD approach using arbitrary 10-mer primers. PCR with the UBC 376 primer generated specific fragments from three species with hairy fruit skin. Those fragments were then cloned to determine their nucleotide sequences. Two SCAR primers were designed from the UBC 376 primer and nucleotide sequences were obtained from the PCR fragments. A SCAR primer, OKC385, specifically amplified a 385-bp fragment from one clone ofActinidia eriantha, four ofActinidia chinensis, and four ofActinidia deliciosa. Deduced amino acid sequences of this fragment showed high sequence homology with plant cellulose synthases, which are involved in the biosynthesis of cellulose, a major cell wall component. The 385-bp fragment was specifically detected only in the seriesPerfectae C.F. Liang of sectionStellatae Li. This type has many hairs on the leaves, fruits, and stems, suggesting that the gene containing the PCR fragment is involved in hair formation in this phylogenetic group. Taken together, our results suggest that the SCAR primer, OKC385, can be used as a specific primer for early selection of the non-hair trait in breeding of the genusActinidia.

키워드

Actinidia; cellulose synthase; RAPD; SCAR primer
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