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Isolation and characterization of a root nodule-specific cysteine proteinase cDNA from soybean

한국식물학회지 2004년 47권 3호 p.216 ~ 216
오창재, 이형석, 김호방, 안정선,
소속 상세정보
오창재 ( Oh Chang-Jae ) - Seoul National University School of Biological Sciences
이형석 ( Lee Hyoung-Seok ) - Seoul National University School of Biological Sciences
김호방 ( Kim Ho-Bang ) - Seoul National University School of Biological Sciences
안정선 ( An Chung-Sun ) - Seoul National University School of Biological Sciences

Abstract


We have determined that a nodule-specific cDNA clone (GmCysP1), obtained from a soybean root nodule-specific EST pool, encodes cysteine proteinase. Its amino acid sequence homology, as well as the conservation of typical motifs and amino acid residues involved in active site formation, shows that GmCysP1 can be classified as a legumain (C13) family cysteine proteinase, belonging to clan CD. Moreover, based on its expression patterns,GmCysP1 is a nodule-specific cysteine proteinase gene that is possibly associated with nodule development or senescence. Our genomic Southern analysis also suggests thatGmCysP1 is a member of a multigene family. Therefore, we propose that GmCysP1 is the first to be identified as a nodule-specific and senescence-related cysteine proteinase that belongs to the legumain family from soybean.

키워드

cysteine proteinase; legumain family; nodule development; senescence; soybean

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