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A study of cotyledon senescence in cucumber (Cucumis sativus L.) based on expressed sequence tags and gene expression

한국식물학회지 2004년 47권 3호 p.244 ~ 253
김대재 ( Kim Dae-Jae ) - Chungbuk National University College of Education School of Science Education

Abstract


Cucumber cotyledons provide an excellent experimental system in which to investigate developmental changes in gene expression, from the early phase of heterotrophism through phototrophic growth to senescence. A cDNA library was prepared from the final stage of senescing cucumber cotyledons (<95% yellow) for studying the genes responsible for lipid mobilization during germination and senescence. This library had produced numerous senescence-associated clones in a previous study. Here, a total of 365 cDNA clones and their expression levels were examined via semi-quantitative RT-PCR. Up-regulation of expression was detected for several known and unknown genes. These results were used to investigate the possible functions for senescence-related genes during cotyledon development

키워드

cotyledon; cucumber; EST; RT-PCR; senescence
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