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Dose-dependent effects of malformin A1 on IAA-induced ethylene production in mung bean (Vigna radiate L.) hypocotyl segments

한국식물학회지 2004년 47권 3호 p.254 ~ 254
김순영 ( Kim Soon-Young ) - Andong National University Department of Biological Sciences

 ( Cho A-Young ) - Andong National University Department of Biological Sciences
 ( Kim Kun-Woo ) - Andong National University School of Bioresource Science
오승은 ( Oh Seung-Eun ) - Konkuk University Department of Biological Sciences

Abstract


Purified malformin A1 (cyclo-D-Cys-D-Cys-L-Val-D-Leu-L-lle), a cyclicpentapeptide toxin fromAspergillus niger, was applied to the hypocotyl segments of mung bean (Vigna radiata L.) seedlings to investigate its role in regulating ethylene biosynthesis. Production of ethylene was induced by treating the plants with 0.1 mM indole-3-acetic acid (1AA). When 0.1 μM malformin A1 was then applied, ethylene production increased and the activities of two key enzymes for its biosynthesis, 1-aminocyclopropane-1-carboxylic acid (ACC)-synthase (ACS) and ACC-oxidase (ACO), were also stimulated. However, at levels of 1 or 10 μM malformin A1, both ethylene production and enzymatic activities were significantly reduced. In the case of ACO,in vitro activity was regulated by malformin A1, independent of ACS activity or the influence of IAA. Furthermore, the conjugate form of ACC, N-malonyl ACC, was significantly promoted by treatment with 0.1 μM malformin A1. These data suggest that malformin A1 can modulate ethylene production through diverse paths and that its effect depends on the concentration of the treatment administered.

키워드

1-aminocyclopropane-1-carboxylic acid; ACC-oxidase; ACC-synthase; lAA-induced ethylene production; N-malonyl; ACC; malformin A1
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