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Micropropagation ofSchizandra chinensis BAILLON using glucose from cotyledonary nodes

한국식물학회지 2004년 47권 3호 p.270 ~ 274
홍민희 ( Hong Min-Hee ) - Chonnam National University Department of Biology

김옥태 ( Kim Ok-Tae ) - Chonnam National University Deptarment of Biology
박재인 ( Park Jae-In ) - Chungbuk National University School of Forest Resources
 ( Hwang Baik ) - Chonnam National University Department of Biology

Abstract


A protocol for the micropropagation ofSchizandra chinensis has been developed using regenerated shoots from axillary bud explants. In preparing to do so, we found that seed type (i.e., mature vs. pre-mature) significantly influenced the rate of germination. The Woody Plant (WP) medium proved to be superior to the Murashige and Skoog (MS) medium for germination purposes. Multiple shoots were induced from cotyledonary nodes of axenic seedlings on WP media containing 6-benzylaminopurine (BA) alone or in combination with 1-napthaleneacetic acid (NAA). High frequencies of shoot proliferation and the greatest number of shoots per explant (11.6) were observed with the use of 1.0 mg L-1 BA. We also established a culture method for proliferating shoots by repeatedly subculturing the original cotyledonary nodes on a shoot multiplication medium each time newly formed shoots were harvested. To induce root formation, glucose was supplied as a carbon-source substitution for sucrose. The best rooting rate was obtained from a WP medium supplemented with 3% glucose and 0.5 mg L-1 NAA. Following transplantation in the field, 82% of the plantlets survived.

키워드

cotyledonary node; glucose; micropropagation; Schizandra chinensis
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