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Selection and characterization of transposon tagging mutants of Synechocystis sp. PCC 6803 sensitive to high-light and oxidative Stresses

한국식물학회지 2004년 47권 4호 p.289 ~ 289
 ( Song Eun-Kyeong ) - Pusan National University Department of Molecular Biology

 ( Zulfugarov Ismayil S. ) - Pusan National University Department of Molecular Biology
김진홍 ( Kim Jin-Hong ) - Pusan National University Department of Molecular Biology
 ( Kim Eun-Ha ) - Pusan National University Department of Molecular Biology
이우성 ( Lee Woo-Sung ) - Sungkyunkwan University Department of Biological Science
이춘환 ( Lee Choon-Hwan ) - Pusan National University Department of Molecular Biology

Abstract


We compared several analytical tools to identify which were most applicable for the selection and characterization of specific transposon-tagged mutant strains ofSynechocystis sp. PCC 6803 that are sensitive to high light and oxidative stresses. Our primary parameter was the maximum photochemical efficiency of dark-adapted cells, a very sensitive factor that can be determined in a non-destructive manner. Using this as a tool for primary selection, we identified five mutant strains with different sensitivities to photoinhibition and photooxidation. For further characterization, we obtained data describing the absorption spectra for pigment contents, the 77K fluorescence spectra, non-photochemical quenching (as a down-regulation process), and the photosynthetic electron transfer rate. Based on these results, we were able to design a strategy for selecting mutants with specific phenotypes. Here, we also discuss the strengths and weaknesses of each selection and characterization tool.

키워드

chlorophyll fluorescence; cyanobacterium; high light; oxidative stress; photoinhibition
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