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Ethylene biosynthesis in a chilling-sensitiveArabidopsis mutant,chs4-2

한국식물학회지 2004년 47권 4호 p.307 ~ 313
 ( Kim Sun-A ) - Ewha Womans University Department of Biological Science

김성기 ( Kim Seong-Ki ) - Chung-Ang University Department of Life Science
 ( Kaufman Peter B. ) - University of Michigan Department of Molecular, Cellular and Developmental Biology
 ( Lee June-Seung ) - Ewha Womans University Department of Biological Science
 ( Chang Soo-Chul ) - Yonsei University University College

Abstract


We investigated chilling-induced changes in ethylene levels in Arabidopsis to find plants with distinct patterns of ethylene production in the cold-related biosynthetic pathway. The sensitive mutants identified here includedchs1-2,chs4-2, andchs6-2. Among these, plants of thechs4-2 mutant produced more ethylene than did the wild type after both were transferred from 4°C or 10°C to 22°C. This mutant also showed less freezing tolerance and more electrolyte leakage than the wild-type plants. Our results suggest a relationship between ethylene biosynthesis and chilling sensitivity in the mutant To determine which of the enzymes involved in ethylene biosynthesis were induced by chilling, we tested the activities of ACC synthase and ACC oxidase in both mutant and wild-type plants, and found greater activity by ACC synthase as well as a higher ACC content in the mutants after all the plants were transferred from 10°C to 22°C. However, ACC oxidase activity did not differ between mutant and wild-type plants in response to chilling treatment Therefore, we conclude thatchs4-2 mutants produce more ethylene than do other mutants or the wild type during their recovery from chilling conditions. Furthermore, we believe that ACC synthase is the key enzyme involved in this response.

키워드

ACC synthase; Arabidopsis; chilling; chs mutant; ethylene
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