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Effect of growth regulators on asiaticoside production in whole plant cultures ofCentella asiatica (L) urban

한국식물학회지 2004년 47권 4호 p.361 ~ 365
김옥태, 김민영, Huh Sun-Mi, Ahn Jun-Cheul, 성낙술, Hwang Baik,
소속 상세정보
김옥태 ( Kim Ok-Tae ) - Chonnam National University Deptarment of Biology
김민영 ( Kim Min-Young ) - Chonnam National University Department of Biology
 ( Huh Sun-Mi ) - Chonnam National University Deptarment of Biology
 ( Ahn Jun-Cheul ) - Seonam University Department of Life Sciences
성낙술 ( Seong Nak-Sul ) - RDA National Institute of Crop Science
 ( Hwang Baik ) - Chonnam National University Department of Biology

Abstract


We investigated the effects of growth regulators on whole-plant cultures derived from nodes ofCentella asiatica. A B5 liquid medium including 0.01 mg L-1 2,4-D resulted in decreased growth and asiaticoside production. Among the cytokinins tested (TDZ, BA, zeatin, and kinetin), TDZ was the best supplement for the promotion of asiaticoside biosynthesis. To directly estimate this effect, we measured asiaticoside content in the leaf, the main organ for synthesis. The addition of TDZ did not affect asiaticoside accumulation. Nevertheless, our results suggest that treatment with exogenous TDZ may enhance the production of asiaticoside in cultures simply through an increase in biomass.

키워드

asiaticoside; Centella asiatica; TDZ; triterpene saponins; whole-plant cultures

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