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Effects of various pretreatments on seed germination ofCalystegia soldanella (Convolvulaceae), a coastal sand dune plant

한국식물학회지 2004년 47권 4호 p.396 ~ 400
 ( Ko Jung-Moon ) - Dankook University Department of Biology

 ( Park Hye-Jeong ) - Dankook University Department of Biology
 ( Min Byeong-Mee ) - Dankook University Department of Science Education
차현철 ( Cha Hyeon-Cheol ) - Dankook University Department of Biology

Abstract


We tested various pretreatments to enhance the germination of ‘Sea bells’ (Calystegia soldanella Roem. et Schult). One experiment, which improved the germination rate by 70%, involved first scarifying the seed coats, then immersing the seeds in 50 ppm GA3 for 24 h. A GA3-alone pretreatment did not increase germination. However, the most effective method, with a 100% success rate, included 3 h of acid pretreatment with 98% H2SO4. We also used scanning electron microscopy (SEM) to examine the seed-coat surfaces and cross-sections of dry seeds. SEM showed structural differences between seeds that were not treated and those exposed to 98% H2SO4. The latter treatment allowed the seed coats to crack and break, thereby disrupting their physical dormancy.

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