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Molecular cloning of a cDNA encoding glutamine synthetase from root nodules ofElaeagnus umbellate

한국식물학회지 2004년 47권 4호 p.401 ~ 406
김호방, Kim Soo-Hyun, 이형석, 오창재, 안정선,
소속 상세정보
김호방 ( Kim Ho-Bang ) - Seoul National University Department of Biological Sciences
 ( Kim Soo-Hyun ) - Seoul National University Department of Biological Sciences
이형석 ( Lee Hyoung-Seok ) - Seoul National University Department of Biological Sciences
오창재 ( Oh Chang-Jae ) - Seoul National University Department of Biological Sciences
안정선 ( An Chung-Sun ) - Seoul National University Department of Biological Sciences

Abstract


We analyzed a cDNA clone encoding cytosolic glutamine synthetase,EuNOD-GS1, isolated from a root nodule cDNA library ofElaeagnus umbellata. This clone has an insert size of 1359 bp and encodes a protein for 355 amino-acid residues, with a molecular weight of 39.2 kDa. Its expression is slightly higher in the root nodules than in the leaves or uninfected roots. Analysis of the deduced amino acid sequences and phytogeny revealed thatEuNOD-GS1 is clustered with cytosolic GS-α isoenzymes. Therefore, based on this and previous results, we propose that the main physiological role ofEuNOD-GS1 is the assimilation of ammonia from secondary and, in part, primary sources.

키워드

cDNA; Elaeagnus umbellata; Frankia; glutamine synthetase; root nodule

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