잠시만 기다려 주세요. 로딩중입니다.

Constitutive expression of two endochitinases from root nodules of Elaeagnus umbellata confers resistance on transgenic Arabidopsis plants against the fungal pathogen Botrytis cinerea

한국식물학회지 2005년 48권 1호 p.39 ~ 46
 ( Kim Yaw-Joo ) - Seoul National University Department of Biological Sciences

김호방 ( Kim Ho-Bang ) - Seoul National University Department of Biological Sciences
 ( Baek Eun-Hye ) - Seoul National University Department of Biological Sciences
허성기 ( Heu Sung-Gi ) - Rural Development Administration National Institute of Agricultural Science and Technology Plant Pathology Division
안정선 ( An Chung-Sun ) - Seoul National University Department of Biological Sciences

Abstract


Plant chitinases have been known as pathogenesis-related (PR) proteins, but recent studies suggest that they play functional roles during normal plant growth and development. We previously isolated two cDNA clones encoding endochitinases,EuNOD-CHT1 and -CHT2, from the root nodules ofElaeagnus umbellata. These genes show differential expression patterns, with theEuNOD-CHT1 gene being active in the root nodules and meristems, whileEuNOD-CHT2 is preferentially expressed in the infected cells of those nodules. To elucidate the functional roles of these two endochitinases, we have now constitutively expressed each gene in a heterologous plant system,Arabidopsis thaliana. Stable inheritance and expression of the transgenes were confirmed by genomic Southern hybridization and RT-PCR. Our transgenic plants did not differ morphologically from the wild types. However, constitutive expression ofEuNOD-CHT1 and -CHT2 inArabidopsis resulted in increased resistance against a fungal pathogen,Botrytis cinerea, but not against a bacterial agent,Pseudomonas syringae pv. Tomato DC3000. Expression levels were enhanced by both wounding and jasmonic acid treatments (forEuNOD-CHT1), or by jasmonic acid only (forEuNOD-CHT2). These data suggest thatEuNOD-CHT1 and -CHT2 primarily play defensive roles during root nodule development inE. umbellata.

키워드

Arabidopsis thaliana; Botrytis cinerea; chitinase; constitutive expression; pathogen resistance; root nodule
원문 및 링크아웃 정보
 
등재저널 정보