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Expression ofMbR4, a TIR-NBS type of appleR Gene, confers resistance to bacterial spot disease inArabidopsis

한국식물학회지 2005년 48권 2호 p.220 ~ 228
이수연 ( Lee Soo-Yeon ) - Ewha Womans University Department of Life Sciences

이동회 ( Lee Dong-Hee ) - Ewha Womans University Department of Life Sciences

Abstract


A single disease resistance gene candidate,MbR4, was isolated from the wild-type apple speciesMalus baccta. This gene was predicted to encode motifs characteristic of the Toll Interleukin 1 Receptor (TIR) ? Nucleotide Binding Site (NBS) of theR gene. Starting with an isolated cDNA clone, genomic clones were obtained via inverse polymerase chain reaction (IPCR). TheMbR4 gene has a single open reading frame (ORF) of 2178 nucleotides, a 41-b untranslated 5’ region, a 21-b untranslated 3’ region, and a predicted protein of 726 amino acids (82 kDa). Its deduced amino acid sequence resembles the N protein of tobacco and the NL25 protein of potato. Ectopic expression ofMbR4 induced enhanced resistance in transgenicArabidopsis plants against the virulent pathogen,Pseudomonas syringae pv.tomato DC3000. Microarray analysis confirmed the induction of defense-related gene expression in pathogen-free 35S::MbR4 heterologousArabidopsis plants, thereby indicating that theMbR4 gene likely activates a pathogen-independent resistance pathway, rather than a gene-for-gene pathway. Our results suggest thatMbR4 plays a role in theR gene, and may be a source of resistance for cultivated apple species.

키워드

apple; disease resistance gene; TIR-NBS (Toll Interleukin 1 Receptor-Nucleotide Binding Site)
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