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Genetic transformation ofAjuga multiflora bunge withAgrobacterium rhizogenes and 20-hydroxyecdysone production in hairy roots

한국식물학회지 2005년 48권 2호 p.258 ~ 262
김옥태 ( Kim Ok-Tae ) - Chonnam National University Deptarment of Biology

 ( Manickavasagm M. ) - Bharathidasan University Tiruchirapalli Department of Biotechnology
 ( Kim Young-Jun ) - Chonnam National University Deptarment of Biology
 ( Jin Mei Ran ) - Chonnam National University Deptarment of Biology
김광수 ( Kim Kwang-Soo ) - Rural Development Administration National Institute of Crop Science
성낙술 ( Seong Nak-Sul ) - RDA National Institute of Crop Science
 ( Hwang Baik ) - Chonnam National University Department of Biology

Abstract


An efficient transformation system forAjuga multiflora Bunge was established by usingAgrobacterium rhizogenes strain A4. After inoculation with the bacteria, we obtained a number of hairy-root clones from micro-calli of the explant petioles. One fast-growing line showed the highest production of 20-hydroxyecdysone (20-HE). PCR amplification of rooting locus (rol) genes revealed that the left hand-transferred DNA of the root-inducing plasmid was inserted into the genome of our transformedAjuga hairy roots. This integration was further confirmed by DNA-DNA hybridization. The 20-HE content in hairy roots was 10 times higher than that measured in the wild type.

키워드

Ajuga multiflora; hairy roots; 20-hydroxyecdysone; Ri-plasmid; rol genes
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