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Isolation and characterization of squalene synthase cDNA fromcentella asiatica (L) urban

한국식물학회지 2005년 48권 3호 p.263 ~ 263
김옥태 ( Kim Ok-Tae ) - RDA National Institute of Crop Science

성낙술 ( Seong Nak-Sul ) - RDA National Institute of Crop Science
김민영 ( Kim Min-Young ) - Chonnam National University Department of Biology
 ( Hwang Baik ) - Chonnam National University Department of Biology

Abstract


We have cloned and characterized a gene for squalene synthase (SQS) fromCentella asiatica (L) Urban, a species that produces a large quantity of triterpene saponins such as asiaticoside and madecassoside. Its full-length cDNA clone was isolated by RACE PCR. The sequence ofpSQS contains an open reading frame of 1248 nucleotides, which code for 416 amino acids with a molecular mass of 47.3 kDa. Southern analysis revealed that one copy might exist in the C.asiatica genome. We also determined that 0.1 mM methyl jasmonate was sufficient to up-regulate those levels ofCaSQS mRNA.

키워드

asiaticoside; Centella asiatica; squalene synthase; triterpene saponins
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