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cDNA cloning and sequence analysis of the rice Cinnamate-4-Hydroxylase gene, a cytochrome P450-dependent monooxygenase involved in the general phenylpropanoid pathway

한국식물학회지 2005년 48권 3호 p.311 ~ 311
양대화 ( Yang Dae-Hwa ) - Korea Atomic Energy Research Institute Division of Radiation Application Research

정병엽 ( Chung Byung-Yeoup ) - Korea Atomic Energy Research Institute Division of Radiation Application Research
 ( Kim Jae-Sung ) - Korea Atomic Energy Research Institute Division of Radiation Application Research
 ( Kim Jin-Hong ) - Korea Atomic Energy Research Institute Division of Radiation Application Research
 ( Yun Pil-Yong ) - Chungnam National University Division of Plant Science and Resources
 ( Lee Young-Ki ) - Chungnam National University Division of Plant Science and Resources
임용표 ( Lim Yong-Pyo ) - Chungnam National University Division of Plant Science and Resources
이명철 ( Lee Myung-Chul ) - Rural Development Administration National Institute of Agricultural Biotechnology

Abstract


Plant cytochrome P450 monooxygenases (P450s) mediate a wide range of oxidative reactions involved in the biosynthesis of phenylpropanoids, terpenes, lipids, and alkaloids. We isolated a cDNA clone for cinnamate-4-hydroxylase (C4H) from a Japonica type rice(Oryza sativa L. cv. llpumbyeo). This C4H has a deduced amino acid sequence that is 85% identical tothat ofSorghum bicolor. Our phylogenetic analysis also showed that theOsC4HL gene is closely related toC4H fromS. bicolor. A putative genomic DNA sequence corresponding toOsC4HL contained cis-elements (boxes P, A, L, and TCA motifs), AT-rich elements, and wound-response elements that control gene expression in its promoter region.OsC4HL expression was detected in all the tissue types, with the highest level being measured in the roots. It was also apparently up-regulated by wounding stress. These data suggest that theOsC4HL gene isC4H member in theCYP73 subfamily.

키워드

C4H; OsC4HL; rice
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