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Effects on GSH synthesis in Chinese cabbage when the culturing solution is supplemented with ammonium sulfate or the constituent amino acids for glutathione

한국식물학회지 2005년 48권 4호 p.404 ~ 410
Kim Ju-Sung, Seo Sang-Gyu, Kim Sun-Hyung, Usui Kenji, Shim Ie-Sung,
소속 상세정보
 ( Kim Ju-Sung ) - University of Tsukuba Graduate School of Life and Environmental Sciences
 ( Seo Sang-Gyu ) - University of Tsukuba Graduate School of Life and Environmental Sciences
 ( Kim Sun-Hyung ) - Ishikawa Prefectural University Research Institute of Natural Resources
 ( Usui Kenji ) - University of Tsukuba Graduate School of Life and Environmental Sciences
 ( Shim Ie-Sung ) - University of Seoul Department of Environmental Horticulture

Abstract


We examined the effects of the constituent amino acids for glutathione (GSH) ? glutamate (Glu), cysteine (Cys), and glycine (Gly) ? on GSH synthesis in Chinese cabbage seedlings. Glu, Cys, and Gly were applied simultaneously (100 mg L-1) to the culture solution for 2 d. When compared with the control, GSH concentrations were increased by 2.1-fold (640.4 nmol g-1 FW) and 1.5-fold (416.4 nmol g-1 FW) in the first leaf and the roots, respectively. Of all the free amino acids, the non-essentials, including Glu, Cys and Gly, occupied 95.5% (shoots) and 81.9% (roots) of the total. Cys supplements greatly enhanced the GSH concentration in the roots; application of 100 mg L-1 increased the level by 7-fold over the control. The activity of GSH synthetase was higher in the roots than in the leaf, whereas that of y-glutamylcysteine synthetase was higher in the leaf.

키워드

Chinese cabbage; cysteine; glutathione; glutathione synthetase; y-glutamylcysteine synthetase

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