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Cloning, characterization, and expression of two cDNA clones for a rice ferulate-5-hydroxylase gene, a cytochrome P450-dependent monooxygenase

한국식물학회지 2006년 49권 3호 p.200 ~ 200
Kim Jin-Hong, 양대화, Kim Jae-Sung, Baek Myung-Hwa, Park Young-Mi, 위승곤, 조재영, 정병엽,
소속 상세정보
 ( Kim Jin-Hong ) - Korea Atomic Energy Research Institute Division of Radiation Application Research
양대화 ( Yang Dae-Hwa ) - Korea Atomic Energy Research Institute Division of Radiation Application Research
 ( Kim Jae-Sung ) - Korea Atomic Energy Research Institute Division of Radiation Application Research
 ( Baek Myung-Hwa ) - Korea Atomic Energy Research Institute Division of Radiation Application Research
 ( Park Young-Mi ) - Korea Atomic Energy Research Institute Division of Radiation Application Research
위승곤 ( Wi Seung-Gon ) - Korea Atomic Energy Research Institute Division of Radiation Application Research
조재영 ( Cho Jae-Young ) - Chonbuk National University Division of Biological Resources Sciences
정병엽 ( Chung Byung-Yeoup ) - Korea Atomic Energy Research Institute Division of Radiation Application Research

Abstract


Ferulate-5-hydroxylase (F5H) is a cytochrome P450-dependent monooxygenase that catalyses the hydroxylation of ferulic acid, coniferaldehyde, and coniferyl alcohol in the pathways leading to sinapic acid and the syringyl unit of lignin. We have isolated twoF5H genes,OsF5HL andOsF5HL2, from a japonica-type rice (Oryza sativa L. cv. llpoombyeo). They are the firstF5H genes experimentally identified in monocotyledonous plants. Phylogenetic analysis indicated that both genes are closely related to dicotF5H genes fromArabidopsis thaliana andBrassica napus. Southern blot analysis showed that these genes exist as single copies in the rice genome. Alignments of theOsF5HL andOsF5HL2 cDNAs to their genomic DNAs revealed thatOsF5HL has an open reading frame (ORF) of 1590 b from four exons, whileOsF5HL2 has an ORF of 1560 b from two exons. Expression ofOsFSHL is highest in young leaves, whereas that ofOsF5HL2 is greatest in mature leaves. In the roots and stems, transcription levels for both genes are markedly low. These data suggest that theOsF5HL andOsF5HL2 genes belong to theCYP84 subfamily and that their expressions are tissue-specific.

키워드

F5H; lignin; OsF5HL; OsF5HL2; rice

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