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Monitoring the occurrence of genetically modified soybean and maize around cultivated fields and at a grain receiving port in Korea

한국식물학회지 2006년 49권 3호 p.218 ~ 223
김창기 ( Kim Chang-Gi ) - Korea Research Institute of Bioscience and Biotechnology Bioevaluation Center

 ( Yi Hoon-Bok ) - Korea Research Institute of Bioscience and Biotechnology Bioevaluation Center
 ( Park Sang-Kyu ) - Ajou University Division of Natural Sciences
 ( Yeon Ji-Eun ) - Korea Research Institute of Bioscience and Biotechnology Bioevaluation Center
김도영 ( Kim Do-Young ) - Korea Research Institute of Bioscience and Biotechnology Bioevaluation Center
김대인 ( Kim Dae-In ) - Korea Research Institute of Bioscience and Biotechnology Bioevaluation Center
 ( Lee Kyu-Hwa ) - Korea Research Institute of Bioscience and Biotechnology Bioevaluation Center
 ( Lee Taek-Chang ) - Korea Research Institute of Bioscience and Biotechnology Bioevaluation Center
 ( Paek In-Soon ) - Korea Research Institute of Bioscience and Biotechnology Bioevaluation Center
윤원기 ( Yoon Won-Kee ) - Korea Research Institute of Bioscience and Biotechnology Bioevaluation Center
정순천 ( Jeong Soon-Chun ) - Korea Research Institute of Bioscience and Biotechnology Bioevaluation Center
김환묵 ( Kim Hwan-Mook ) - Korea Research Institute of Bioscience and Biotechnology Bioevaluation Center

Abstract


Increased imports of genetically modified (CM) soybean and maize might cause genetic contamination of those crops that are conventionally bred, as well as wild soybeans within Korea. Leaves of maize and both cultivated and wild soybeans were sampled in and near rural fields to detect the presence of transgenes. Roadsides around a major grain port in Incheon were also surveyed to monitor the occurrence of incoming CM soybean and maize. The amplificability of DNA extracted from the collected samples was determined by PCR using soybean- or maize-specific primers: lectin and zein genes, respectively. The presence or absence of transgenes was detected by primer sets for the 35S and nos genes. Transgenes were not found in the cultivated or wild soybean or in the maize collected from cultivated fields. However, we obtained one GM maize plant among seven along the roadsides around Incheon Port. Although the effect of a single GM maize plant would be negligible and would not pose any threat to natural environments, an increase in the import of GM plants might lead to future, unapproved cultivation of GM crops. Therefore, appropriate monitoring is necessary to detect the occurrence of GM plants in areas around grain receiving ports and within agroecosystems.

키워드

detection; genetically modified plant; maize; monitoring; PCR; soybean
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